Lysozyme Sorption in Hydrogel Contact Lenses

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dc.contributor.author Garrett, Qian en_US
dc.contributor.author Garrett, R en_US
dc.contributor.author Milthorpe, Bruce en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:50:12Z
dc.date.available 2010-05-28T09:50:12Z
dc.date.issued 1999 en_US
dc.identifier 2008004467 en_US
dc.identifier.citation Garrett Qian, Garrett R, and Milthorpe Bruce 1999, 'Lysozyme Sorption in Hydrogel Contact Lenses', Association for Research in Vision and Ophthalmology, vol. 40, no. 5, pp. 897-903. en_US
dc.identifier.issn 0146-0404 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/9520
dc.description.abstract To examine the processes involved in formation of protein deposits on hydrogel contact lenses. METHODS: The adsorption and/or penetration of lysozyme on or into three types of contact lenses, etafilcon A, vifilcon A, and tefilcon, were investigated in vitro using a radiolabel-tracer technique, x-ray photoelectron spectroscopy, and laser scanning confocal microscopy. RESULTS: Binding of lysozyme to high-water-content, ionic contact lenses (etafilcon A and vifilcon A) was dominated by a penetration process. The extent of this penetration was a function of charge density of the lenses, so that there was a higher degree of penetration of lysozyme in etafilcon A than in vifilcon A lenses. In contrast, the binding of lysozyme to tefilcon lenses was a surface adsorption process. The adsorption and desorption kinetics showed similar trends to those found in human serum albumin (HSA) adsorption on lens surfaces. However, the extent of lysozyme adsorption on tefilcon is much higher than HSA adsorption, probably because of the self-association of lysozyme on the tefilcon lens surface. Furthermore, either penetration or adsorption of lysozyme involved reversible and irreversible processes and were both time dependent. CONCLUSIONS: Binding of lysozyme to hydrogel lenses involves surface adsorption or matrix penetration. These processes may be reversible or irreversible. The properties of the lens materials, such as charge density (ionicity) and porosity (water content) of the lenses, determine the type and rates of these processes. en_US
dc.language en_US
dc.publisher Association for Research in Vision and Ophthalmology en_US
dc.title Lysozyme Sorption in Hydrogel Contact Lenses en_US
dc.parent Investigative Ophthalmology & Visual Science en_US
dc.journal.volume 40 en_US
dc.journal.number 5 en_US
dc.publocation USA en_US
dc.identifier.startpage 897 en_US
dc.identifier.endpage 903 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110800 en_US
dc.personcode 0000049879 en_US
dc.personcode 0000049884 en_US
dc.personcode 105631 en_US
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US
dc.staffid 105631 en_US


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