Growth-promoting effect of Rh(D) antibody on human pancreatic islet cells

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Show simple item record Feller, John en_US Simpson, Ann en_US Nelson, Margaret en_US Swan, M en_US O'Connell, Philip en_US Hawthorne, Wayne en_US Tao, Chang en_US O'Brien, Bronwyn en_US
dc.contributor.editor en_US 2010-05-28T09:49:38Z 2010-05-28T09:49:38Z 2008 en_US
dc.identifier 2007005021 en_US
dc.identifier.citation Feller John et al. 2008, 'Growth-promoting effect of Rh(D) antibody on human pancreatic islet cells', The Endocrine Society, vol. 93, no. 9, pp. 3560-3567. en_US
dc.identifier.issn 0021-972X en_US
dc.identifier.other C1 en_US
dc.description.abstract Context/Objective: Hyperinsulinism with islet cell hyperplasia is a frequent complication, of unknown cause, in hemolytic disease of the newborn, occurring in Rh(D)-positive infants of Rh-isoimmunized Rh(D)-negative mothers, but not in infants with other hemolytic disorders. We investigated the possibility that trans-placentally acquired anti-D Ig is the cause of both conditions. Design: Monolayer cultures of human islet cells were exposed to sera from Rh-isoimmunized mothers and newborns, where jaundice, hyperinsulinism, and hypoglycemia in the infant had ensued. Parallel cultures with anti-D, specific anti-D monoclonal antibodies, normal human Ig (15 I?g/ml), and serum controls were also undertaken. Islet cell proliferation was determined by [ 3H]thymidine incorporation. Insulin storage and chronic and acute insulin secretion to glucose were analyzed by RIA. Rh(D) surface antigen expression was determined on islet cells by flow cytometric analysis. Results: Islet cell proliferation and insulin secretion were significantly greater in coculture with test sera (P < 0.01; n = 8) and with anti-D (P < 0.001; n = 8), compared with either controls or Ig. After 8 d of growth, the static incubation experiment showed a 3.5-fold response to glucose stimulus in all sera. Rh(D) antigen expression was detected on the islet cell surface by flow cytometry, and islet cell morphology was normal. Colocalization of the proliferation marker Ki67 with insulin by immunofluorescent staining further indicated that Rh(D) antibody promoted islet growth. Conclusions: The anti-Rh(D) islet cell proliferative effect generates neonatal hyperinsulinism in Rh isoimmunization. Anti-Rh(D) may have application for islet cell proliferation in diabetes mellitus treatment for Rh(D)-positive subjects. Further analysis is required. Copyright ? 2008 by The Endocrine Society. en_US
dc.language en_US
dc.publisher The Endocrine Society en_US
dc.relation.isbasedon en_US
dc.title Growth-promoting effect of Rh(D) antibody on human pancreatic islet cells en_US
dc.parent Journal of Clinical Endocrinology and Metabolism en_US
dc.journal.volume 93 en_US
dc.journal.number 9 en_US
dc.publocation USA en_US
dc.identifier.startpage 3560 en_US
dc.identifier.endpage 3567 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 111400 en_US
dc.personcode 0000046616 en_US
dc.personcode 940084 en_US
dc.personcode 0000046618 en_US
dc.personcode MSWAN en_US
dc.personcode 0000046619 en_US
dc.personcode 0000046620 en_US
dc.personcode 010036 en_US
dc.personcode 030027 en_US
dc.percentage 100 en_US Paediatrics and Reproductive Medicine en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords en_US
dc.staffid 030027 en_US

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