Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures

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dc.contributor.author Miller, Diana en_US
dc.contributor.author Grimwade, Julia en_US
dc.contributor.author Betteridge, Thu en_US
dc.contributor.author Rozgaja, Tania en_US
dc.contributor.author Torgue, Julien en_US
dc.contributor.author Leonard, Alan en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:49:36Z
dc.date.available 2010-05-28T09:49:36Z
dc.date.issued 2009 en_US
dc.identifier 2008008310 en_US
dc.identifier.citation Miller Diana et al. 2009, 'Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures', National Academy of Sciences, vol. 106, no. 44, pp. 18479-18484. en_US
dc.identifier.issn 0027-8424 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/9437
dc.description.abstract Eukaryotic initiator proteins form origin recognition complexes (ORCs) that bind to replication origins during most of the cell cycle and direct assembly of prereplication complexes (pre-RCs) before the onset of S phase. In the eubacterium Escherichia coli, there is a temporally similar nucleoprotein complex comprising the initiator protein DnaA bound to three high-affinity recognition sites in the unique origin of replication, oriC. At the time of initiation, this high-affinity DnaA?oriC complex (the bacterial ORC) accumulates additional DnaA that interacts with lower-affinity sites in oriC, forming a pre-RC. In this paper, we investigate the functional role of the bacterial ORC and examine whether it mediates low-affinity DnaA?oriC interactions during pre-RC assembly. We report that E. coli ORC is essential for DnaA occupation of low-affinity sites. The assistance given by ORC is directed primarily to proximal weak sites and requires oligomerization-proficient DnaA. We propose that in bacteria, DnaA oligomers of limited length and stability emerge from single high-affinity sites and extend toward weak sites to facilitate their loading as a key stage of prokaryotic pre-RC assembly. en_US
dc.language en_US
dc.publisher National Academy of Sciences en_US
dc.relation.isbasedon http://dx.doi.org/10.1073/pnas.0909472106 en_US
dc.title Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures en_US
dc.parent Proceedings Of The National Academy Of Sciences ... en_US
dc.journal.volume 106 en_US
dc.journal.number 44 en_US
dc.publocation USA en_US
dc.identifier.startpage 18479 en_US
dc.identifier.endpage 18484 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000053902 en_US
dc.personcode 0000053903 en_US
dc.personcode 106539 en_US
dc.personcode 0000053904 en_US
dc.personcode 0000053905 en_US
dc.personcode 0000053906 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords DNA replication, E. coli, ORC, oriC, pre-RC en_US


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