Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns

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dc.contributor.author Eamens, G en_US
dc.contributor.author Forbes, W en_US
dc.contributor.author Djordjevic, Steven en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:56Z
dc.date.available 2010-05-28T09:45:56Z
dc.date.issued 2006 en_US
dc.identifier 2008007043 en_US
dc.identifier.citation Eamens G, Forbes W, and Djordjevic Steven 2006, 'Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns', Elsevier BV, vol. 115, pp. 329-338. en_US
dc.identifier.issn 0378-1135 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8859
dc.description.abstract Swine erysipelas vaccines are routinely used to protect pigs against peracute and acute/urticarial forms of Erysipelothrix. Between 1995 and 1998, 34 swine herds across four Australian states experienced vaccine failure. Forty-four isolates of Erysipelothrix rhusiopathiae of serovars 2, 1a, 1b and 1b ? 21 were recovered from 15 of these 34 vaccine breakdown herds. These isolates were characterised by restriction fragment length polymorphism (RFLP) analyses using RsaI and AluI on whole cell DNA and for the presence of plasmid DNA. Results were compared with those of 20 isolates from 16 herds unaffected by vaccine breakdown and 13 isolates representing 10 reference strains. The majority of breakdown herds possessed isolates of serovar 2 (9/15 herds), followed by serovar 1a (5 herds). No geographic predominance of a single serovar was evident. The identification of 10 RsaI profiles from whole cell DNA among the 44 isolates from 15 breakdown herds indicated that a single, new clonal lineage of E. rhusiopathiae was not responsible for vaccine failure. RsaI RFLP analyses detected a further 14 distinct profiles among 20 field strains unassociated with vaccine breakdowns, and none matched profiles of the 10 serovar reference strains for serovars 1a, 1b, 2 or 21. This technique is recommended for epidemiological studies of E. rhusiopathiae strains. en_US
dc.language en_US
dc.publisher Elsevier BV en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.vetmic.2006.02.015 en_US
dc.title Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns en_US
dc.parent Veterinary Microbiology en_US
dc.journal.volume 115 en_US
dc.journal.number en_US
dc.journal.number 4 en_US
dc.publocation Netherlands en_US
dc.identifier.startpage 329 en_US
dc.identifier.endpage 338 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 070707 en_US
dc.personcode 0000052262 en_US
dc.personcode 0000052263 en_US
dc.personcode 107126 en_US
dc.percentage 100 en_US
dc.classification.name Veterinary Microbiology (excl. Virology) en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Erysipelothrix rhusiopathiae; Restriction fragment length polymorphism; Plasmids en_US
dc.staffid en_US
dc.staffid 107126 en_US


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