Challenge of New Zealand Greenshell (TM) mussel Perna canaliculus larvae using two Vibrio pathogens: a hatchery study

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Show simple item record Kesarcodi-Watson, Aditya en_US Kaspar, Heinrich en_US Lategan, Maria en_US Gibson, Lewis en_US
dc.contributor.editor en_US 2010-05-28T09:45:53Z 2010-05-28T09:45:53Z 2009 en_US
dc.identifier 2008007751 en_US
dc.identifier.citation Kesarcodi-Watson Aditya et al. 2009, 'Challenge of New Zealand Greenshell (TM) mussel Perna canaliculus larvae using two Vibrio pathogens: a hatchery study', Inter-Research, vol. 86, no. 1, pp. 15-20. en_US
dc.identifier.issn 0177-5103 en_US
dc.identifier.other C1 en_US
dc.description.abstract Bacterial diseases remain a large problem in aquaculture hatcheries. The successful design and implementation of protective measures in the hatchery depends on an understanding of the dynamics of the infection process. Developing an in situ experimental protocol for pathogen challenge is therefore of paramount importance. Here, we demonstrated the minimum effective pathogenic dose (MEPD) of Vibrio splendidus (10(5) CFU ml(-1)) and a Vibrio coralliilyticus/neptunius-like isolate, Vibrio sp. DO1 (10(6) CFU ml(-1)), for New Zealand Greenshell (TM) mussel (GSM, Perna canaliculus) larvae during hatchery production. In a flow-through water hatchery system, larvae given 1 to 2 h of static water exposure to these pathogen doses showed respective average cumulative mortalities of 58 and 69 % on the fourth day following pathogen exposure. After the 1 to 2 h static exposure, larvae were returned to flow-through water. Larvae exposed to a dosage one order of magnitude greater than the MEPD had higher mortalities of 73 and 96 % for V. splendidus and Vibrio sp. DO1 respectively. These 4 levels of mortality were significantly greater than those of the non-exposed control larvae which respectively averaged 23 and 35 % in experiments involving V splendidus and Vibrio sp. DO1. Experiments were repeated 4 times to confirm reproducibility. After pathogen exposure, pathogens were detected in the larvae and tank water of treatments with dosages of >= 10(5) CFU ml-1 (V. splendidus) and 10(6) CFU ml(-1) (Vibrio sp. DO1), but not in treatments with lower pathogen dosages. The challenge protocols are reproducible and provide an opportunity to assess measures for the protection of GSM larvae against infection in the hatchery environment. en_US
dc.language en_US
dc.publisher Inter-Research en_US
dc.title Challenge of New Zealand Greenshell (TM) mussel Perna canaliculus larvae using two Vibrio pathogens: a hatchery study en_US
dc.parent Disease of Aquatic Organisms en_US
dc.journal.volume 86 en_US
dc.journal.number 1 en_US
dc.publocation Oldendorf Luhe en_US
dc.identifier.startpage 15 en_US
dc.identifier.endpage 20 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 070404 en_US
dc.personcode 10263681 en_US
dc.personcode 0000049645 en_US
dc.personcode 004569 en_US
dc.personcode 102957 en_US
dc.percentage 100 en_US Fish Pests and Diseases en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity ISI:000270802000003 en_US
dc.description.keywords Vibriosis; Challenge protocol; Greenshell (TM) mussel; Larviculture en_US
dc.staffid en_US
dc.staffid 102957 en_US

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