A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype

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dc.contributor.author Robinson, Andrew en_US
dc.contributor.author Guilfoyle, Amy en_US
dc.contributor.author Harrop, Stephen en_US
dc.contributor.author Boucher, Yan en_US
dc.contributor.author Curmi, Paul en_US
dc.contributor.author Mabbutt, Bridget en_US
dc.contributor.author Stokes, Hatch en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:46Z
dc.date.available 2010-05-28T09:45:46Z
dc.date.issued 2007 en_US
dc.identifier 2008005554 en_US
dc.identifier.citation Robinson Andrew et al. 2007, 'A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype', Blackwell Publishing Ltd, vol. 66, no. 3, pp. 610-621. en_US
dc.identifier.issn 0950-382X en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8840
dc.description.abstract Mobile gene cassettes collectively contain a highly diverse pool of novel genes that encode many novel adaptive functions. In the non-clinical context, the function of almost all of the encoded proteins remains unknown despite the enormous size of this mobile gene pool. We have been characterizing cassette arrays by taking advantage of the fact that they cluster at discrete sites in chromosomes; even large arrays are thus recoverable in a relatively small number of clones in genomic libraries. In one assembled array of 116 cassettes from the marine bacterium Vibrio sp. DAT722, a putative MazG protein is encoded within the 21st cassette. Because MazG proteins are implicated in a number of cellular processes, including house-cleaning and stress survival, the presence of such a protein in a mobile cassette was noteworthy. Here we solve the crystal structure of this ?-helical protein, and define both open and closed states of a new variant of the MazG family. Functional assays confirm that the protein is a dNTP pyrophosphohydrolase, with marked preferences for dCTP and dATP. We hypothesize that iMazG acts as a house-cleaning enzyme, preventing the incorporation of damaging non-canonical nucleotides into host-cell DNA. en_US
dc.language en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1365-2958.2007.05932.x en_US
dc.title A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype en_US
dc.parent Molecular Microbiology en_US
dc.journal.volume 66 en_US
dc.journal.number 3 en_US
dc.publocation UK en_US
dc.identifier.startpage 610 en_US
dc.identifier.endpage 621 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000050593 en_US
dc.personcode 0000050594 en_US
dc.personcode 0000030015 en_US
dc.personcode 0000050228 en_US
dc.personcode 105741 en_US
dc.personcode 0000030026 en_US
dc.personcode 0000050529 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US

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