Use of the chromosomal integron array as a phylogenetic typing system for Vibrio cholerae pandemic strains

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Show simple item record Labbate, Maurizio en_US Boucher, Yan en_US Joss, M en_US Michael, C en_US Gillings, Michael en_US Stokes, Hatch en_US
dc.contributor.editor en_US 2010-05-28T09:45:45Z 2010-05-28T09:45:45Z 2007 en_US
dc.identifier 2008003900 en_US
dc.identifier.citation Labbate Maurizio et al. 2007, 'Use of the chromosomal integron array as a phylogenetic typing system for Vibrio cholerae pandemic strains', The Society for General Microbiology, vol. 153, no. 5, pp. 1488-1498. en_US
dc.identifier.issn 1350-0872 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Approximately 200 serogroups of Vibrio cholerae exist, with only two, O1 and O139, responsible for epidemic and pandemic cholera. Strains from these serogroups have evolved from a common progenitor, with lateral gene transfer largely driving their emergence. These strains are so closely related that separation using single- or multi-locus phylogeny has proven difficult. V. cholerae strains contain a genetic system called the integron that is located in the chromosome and that can integrate and excise DNA elements called mobile gene cassettes (MGCs) by site-specific recombination. Large arrays of MGCs are found in V. cholerae strains. For instance, the O1 El Tor strain N16961 contains 179 MGCs. Since integron arrays are dynamic through recombination and excision of MGCs, it was hypothesized that the MGC composition in a given V. cholerae pandemic strain would be useful as a phylogenetic typing system. To address this, a PCR-based method was used to rapidly characterize the MGC composition of V. cholerae arrays. The results showed that the MGC composition of pandemic V. cholerae cassette arrays is relatively conserved, providing further evidence that these strains have evolved from a common progenitor. Comparison of MGC composition between the V. cholerae pandemic strains was also able to resolve the evolution of O139 from a subgroup of O1 El Tor. This level of differentiation of closely related V. cholerae isolates was more sensitive than conventional single-gene phylogeny or multi-locus sequence analysis. Using this method, novel MGCs from an O1 classical strain and an Argentinian O139 isolate were also identified, and a major deletion in the MGC array in all pandemic O139 strains and a subset of O1 El Tor strains was identified. Analysis of sequenced V. cholerae integron arrays showed that their evolution can proceed by rearrangements and deletions/insertions of large portions of MGCs in addition to the insertion or excision of single MGCs. en_US
dc.language en_US
dc.publisher The Society for General Microbiology en_US
dc.relation.isbasedon en_US
dc.title Use of the chromosomal integron array as a phylogenetic typing system for Vibrio cholerae pandemic strains en_US
dc.parent Microbiology-uk en_US
dc.journal.volume 153 en_US
dc.journal.number 5 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 1488 en_US
dc.identifier.endpage 1498 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060503 en_US
dc.personcode 106011 en_US
dc.personcode 0000050228 en_US
dc.personcode 0000050229 en_US
dc.personcode 0000050230 en_US
dc.personcode 0000030144 en_US
dc.personcode 105741 en_US
dc.percentage 100 en_US Microbial Genetics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords 59-be, 59-base element; 6-FAM, 6-carboxyfluorescein; gDNA, genomic DNA; LGT, lateral gene transfer; MGC, mobile gene cassette; VCR, V. cholerae repeat sequence en_US
dc.staffid 105741 en_US

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