In vitro transposon mutagenesis of an equine herpesvirus 1 genome cloned as a bacterial artificial chromosome

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In vitro transposon mutagenesis of an equine herpesvirus 1 genome cloned as a bacterial artificial chromosome

Hansen K; Napier Ian; Koen M; Bradford Sharon; Messerle M; Bell Emma; Seshadri L; Stokes Harold; Birch Debra; Whalley J
URI: http://hdl.handle.net/10453/8836
Date: 2006

Abstract:

The 150-kbp genome of the alphaherpesvirus equine herpesvirus 1 (EHV-1) strain HVS25A was cloned as a bacterial artificial chromosome (EHV-1 BAC), with mini F plasmid sequences inserted between genes 62 and 63. Transfection of EHV-1 BAC DNA purified from

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