Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

UTSePress Research/Manakin Repository

Search UTSePress Research


Advanced Search

Browse

My Account

Show simple item record

dc.contributor.author Barken, Kb en_US
dc.contributor.author Pamp, Sj en_US
dc.contributor.author Yang, L en_US
dc.contributor.author Gjermansen, M en_US
dc.contributor.author Bertrand, Jacob en_US
dc.contributor.author Klausen, Mikkel en_US
dc.contributor.author Givskov, Michael en_US
dc.contributor.author Whitchurch, Cynthia en_US
dc.contributor.author Engel, Joanne en_US
dc.contributor.author Tolker-Nielsen, Tim en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:36Z
dc.date.available 2010-05-28T09:45:36Z
dc.date.issued 2008 en_US
dc.identifier 2008006317 en_US
dc.identifier.citation Barken Kb et al. 2008, 'Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms', Blackwell Publishing, vol. 10, no. 9, pp. 2331-2343. en_US
dc.identifier.issn 1462-2912 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8814
dc.description.abstract When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms. en_US
dc.language en_US
dc.publisher Blackwell Publishing en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1462-2920.2008.01658.x en_US
dc.title Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms en_US
dc.parent Environmental Microbiology en_US
dc.journal.volume 10 en_US
dc.journal.number 9 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 2331 en_US
dc.identifier.endpage 2343 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000051661 en_US
dc.personcode 0000051662 en_US
dc.personcode 0000101149 en_US
dc.personcode 0000051664 en_US
dc.personcode 0000047372 en_US
dc.personcode 0000051665 en_US
dc.personcode 0000050243 en_US
dc.personcode 103745 en_US
dc.personcode 0000047386 en_US
dc.personcode 0000047380 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000258203500013 en_US
dc.description.keywords NA en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record