New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase

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New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase

Nield Blair; Willows Robert; Torda Andrew; Gillings Michael; Holmes A; Nevalainen Kmh; Stokes Harold; Mabbutt Bridget
URI: http://hdl.handle.net/10453/8759
Date: 2004

Abstract:

By targeting gene cassettes by polymerase chain reaction (PCR) directly from environmentally derived DNA, we are able to amplify entire open reading frames (ORFs) independently of prior sequence knowledge. Approximately 10% of the mobile genes recovered

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