Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid

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dc.contributor.author Betteridge, Thu en_US
dc.contributor.author Yang, J. en_US
dc.contributor.author Pittard, A. en_US
dc.contributor.author Praszkier, J. en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:15Z
dc.date.available 2010-05-28T09:45:15Z
dc.date.issued 2004 en_US
dc.identifier 2008008687 en_US
dc.identifier.citation Betteridge Thu et al. 2004, 'Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid', Amer Soc Microbiology, vol. 186, no. 12, pp. 3785-3793. en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8754
dc.description.abstract The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5'-TCTTAA-3', which lies within the opened region, was essential for the localized unwinding of the origin in vitro and origin activity in vivo. In addition, efficient unwinding of the origin of replication of pMU720 in vitro required the native positioning of the binding sites for the initiator proteins. Interestingly, binding of RepA to RepA box 1, which is essential for origin activity, was not required for the localized opening of the origin in vitro. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.relation.isbasedon http://dx.doi.org/10.1128/JB.186.12.3785-3793.2004 en_US
dc.title Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid en_US
dc.parent Journal Of Bacteriology en_US
dc.journal.volume 186 en_US
dc.journal.number 12 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 3785 en_US
dc.identifier.endpage 3793 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060503 en_US
dc.personcode 106539 en_US
dc.personcode 0000054003 en_US
dc.personcode 0000054004 en_US
dc.personcode 0000054005 en_US
dc.percentage 50 en_US
dc.classification.name Microbial Genetics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US


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