The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR

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dc.contributor.author Mckee, Ben en_US
dc.contributor.author Djordjevic, Steven en_US
dc.contributor.author Goodman, Russell en_US
dc.contributor.author Hornitzky, Michael en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:13Z
dc.date.available 2010-05-28T09:45:13Z
dc.date.issued 2003 en_US
dc.identifier 2008007203 en_US
dc.identifier.citation Mckee Ben et al. 2003, 'The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR', E D P Sciences, vol. 34, pp. 19-27. en_US
dc.identifier.issn 0044-8435 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8747
dc.description.abstract A hemi-nested polymerase chain reaction (PCR) was further developed for the detection of Melissococcus pluton in adult bees and honey bee products. A chloroform:isoamyl alcohol DNA extraction method was used to provide template from 154 samples of adult bee tissues, honey, pollen, whole larvae and comb cells. All 36 honey bee samples tested from a diseased colony were shown to contain M. pluton and sub-clinical infections were detected in adult bee tissues, larvae and honey (49/98; 50.0%) collected from all 9 healthy colonies from areas where EFB was endemic. All 20 adult bee tissue samples from a healthy colony from Western Australia where EFB has never been reported were negative. Of 80 bulk honey samples from six Australian states, 55 of 80 (68.8%) samples were shown to contain M. pluton whereas culture techniques detected M. pluton in 22 of 80 (27.5%) of these samples. M. pluton was detected in honey from all Australian states except Western Australia. en_US
dc.language en_US
dc.publisher E D P Sciences en_US
dc.relation.isbasedon http://dx.doi.org/10.1051/apido:2002047 en_US
dc.title The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR en_US
dc.parent Apidologie en_US
dc.journal.volume 34 en_US
dc.journal.number en_US
dc.publocation France en_US
dc.identifier.startpage 19 en_US
dc.identifier.endpage 27 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060800 en_US
dc.personcode 0000052468 en_US
dc.personcode 107126 en_US
dc.personcode 0000052469 en_US
dc.personcode 0000052309 en_US
dc.percentage 100 en_US
dc.classification.name Zoology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US
dc.staffid en_US


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