Definition of the attI1 site of class 1 integrons

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dc.contributor.author Partridge, S en_US
dc.contributor.author Recchia, Gavin en_US
dc.contributor.author Scaramuzzi, Carol en_US
dc.contributor.author Collis, Christina en_US
dc.contributor.author Hall, Ruth en_US
dc.contributor.author Stokes, Hatch en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:45:09Z
dc.date.available 2010-05-28T09:45:09Z
dc.date.issued 2000 en_US
dc.identifier 2008005390 en_US
dc.identifier.citation Partridge S et al. 2000, 'Definition of the attI1 site of class 1 integrons', Society for General Microbiology, vol. 146, pp. 2855-2864. en_US
dc.identifier.issn 1350-0872 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8735
dc.description.abstract Integron-encoded integrases recognize two distinct types of recombination site: attI sites, found in integrons, and members of the 59-base element (59-be) family, found in the integron-associated gene cassettes. The class 1 integron integrase, IntI1, catalyses recombination between attI1 and a 59-be, two 59-be, or two attI1 sites, but events involving two attI1 sites are less efficient than the reactions in which a 59-be participates. The full attI1 site is required for high-efficiency recombination with a 59-be site. It is 65 bp in length and includes a simple site, consisting of a pair of inversely oriented IntI1-binding domains, together with two further directly oriented IntI1-binding sites designated strong and weak. However, a smaller region that contains only the simple site is sufficient to support a lower level of recombination with a complete attI1 partner and the features that determine the orientation of attI1 reside within this region. An unusual reaction between the attI1 site and a 59-be appears to be responsible for the loss of the central region of a 59-be to create a potential fusion of two adjacent gene cassettes. en_US
dc.language en_US
dc.publisher Society for General Microbiology en_US
dc.relation.isbasedon NA en_US
dc.title Definition of the attI1 site of class 1 integrons en_US
dc.parent Microbiology-uk en_US
dc.journal.volume 146 en_US
dc.journal.number en_US
dc.journal.number 1 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 2855 en_US
dc.identifier.endpage 2864 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000033693 en_US
dc.personcode 0000021502 en_US
dc.personcode 0000050522 en_US
dc.personcode 0000050523 en_US
dc.personcode 105741 en_US
dc.personcode 0000023445 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords attI, integron, integrase, site-speci?c recombination, gene cassettes en_US


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