Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil

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Show simple item record Holmes, Andrew en_US Holley, Marita en_US Mahon, Andrew en_US Nield, Blair en_US Gillings, Michael en_US Stokes, Hatch en_US
dc.contributor.editor en_US 2010-05-28T09:45:00Z 2010-05-28T09:45:00Z 2003 en_US
dc.identifier 2008005541 en_US
dc.identifier.citation Holmes Andrew et al. 2003, 'Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil', Amer Soc Microbiology, vol. 185, no. 3, pp. 918-928. en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Class 1 integrons have strongly influenced the evolution of multiple antibiotic resistance. Diverse integrons have recently been detected directly in a range of natural environments. In order to characterize the properties of these environmental integrons, we sought to isolate organisms containing integrons from soils, which resulted in the isolation of Pseudomonas stutzeri strain Q. Further isolation efforts targeted at this species resulted in recovery of two other strains (P and BAM). 16S rRNA sequences and chromosome mapping showed that these three strains are very closely related clonal variants in a single genomovar of P. stutzeri. Only strains Q and BAM were found to contain an integron and an associated gene cassette array. The intI and attI components of these strains showed 99 and 90% identity, respectively. The structure of these integrons and their associated gene cassettes was similar to that reported previously for other integron classes. The two integrons contained nonoverlapping sets of cassette-associated genes. In contrast, many of the cassette-associated recombination sites in the two integrons were similar and were considered to constitute a distinct subfamily consisting of 59-base element (59-be) recombination sites (the Pseudomonas subfamily). The recombination activity of P. stutzeri integron components was tested in cointegrate assays. IntIPstQ was shown to catalyze site-specific recombination between its cognate attI site and 59-be sites from antibiotic resistance gene cassettes. While IntIPstQ did not efficiently mediate recombination between members of the Pseudomonas 59-be subfamily and other 59-be types, the former sites were functional when they were tested with IntI1. We concluded that integrons present in P. stutzeri possess recombination activity and represent a hot spot for genomic diversity in this species. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.relation.isbasedon en_US
dc.title Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil en_US
dc.parent Journal Of Bacteriology en_US
dc.journal.volume 185 en_US
dc.journal.number 3 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 918 en_US
dc.identifier.endpage 928 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060503 en_US
dc.personcode 0000050528 en_US
dc.personcode 0000033088 en_US
dc.personcode 0000050584 en_US
dc.personcode 104673 en_US
dc.personcode 0000030144 en_US
dc.personcode 105741 en_US
dc.percentage 100 en_US Microbial Genetics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid 105741 en_US

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