Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies

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Show simple item record Cordwell, Stuart en_US Len, Alice en_US Touma, Rachel en_US Scott, Nichollas en_US Jones, David en_US Connolly, Angela en_US Crossett, Ben en_US Djordjevic, Steven en_US Falconer, Linda en_US
dc.contributor.editor en_US 2010-05-28T09:44:59Z 2010-05-28T09:44:59Z 2008 en_US
dc.identifier 2008007075 en_US
dc.identifier.citation Cordwell Stuart et al. 2008, 'Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies', Wiley - V C H Verlag GmbH & Co. KGaA, vol. 8, pp. 122-139. en_US
dc.identifier.issn 1615-9853 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Campylobacter jejuni is the leading cause of food- and water-borne illness world-wide. The membrane-associated proteome of a recent C. jejuni gastrointestinal isolate (JHH1) was generated by sodium carbonate precipitation and ultracentrifugation followed by 2-DE and MALDI-TOF MS as well as 2-DLC (strong cation exchange followed by RP chromatography) of trypsin digests coupled to MS/MS (2-DLC/MS/MS). 2-DE/MS identified 77 proteins, 44 of which were predicted membrane proteins, while 2-DLC/MS/MS identified 432 proteins, of which 206 were predicted to be membrane associated. A total of 453 unique proteins (27.4% of the C. jejuni theoretical proteome), including 187 bona fide membrane proteins were identified in this study. Membrane proteins were also compared between C. jejuni JHH1 and ATCC 700297 to identify factors potentially associated with increased gastrointestinal virulence. We identified 28 proteins that were significantly (>two-fold) more abundant in, or unique to, JHH1, including eight proteins involved in chemotaxis signal transduction and flagellar motility, the amino acid-binding surface antigens CjaA and CjaC, and four outer membrane proteins (OMPs) of unknown function (Cj0129c, Cj1031, Cj1279c, and Cj1721c). Immunoblotting using convalescent patient sera generated postgastrointestinal infection revealed 13 (JHH1) and 12 (ATCC 700297) immunoreactive proteins. These included flagellin (FlaA) and CadF as well as Omp18, Omp50, Cj1721c, PEB1A, PEB2, and PEB4A. This study provides a comprehensive analysis of membrane-associated proteins from C. jejuni. en_US
dc.language en_US
dc.publisher Wiley - V C H Verlag GmbH & Co. KGaA en_US
dc.relation.isbasedon en_US
dc.title Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies en_US
dc.parent Proteomics en_US
dc.journal.volume 8 en_US
dc.journal.number en_US
dc.journal.number 1 en_US
dc.publocation Weinheim, Germany en_US
dc.identifier.startpage 122 en_US
dc.identifier.endpage 139 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000027041 en_US
dc.personcode 0000052333 en_US
dc.personcode 0000052334 en_US
dc.personcode 0000052321 en_US
dc.personcode 0000052335 en_US
dc.personcode 0000052323 en_US
dc.personcode 0000052336 en_US
dc.personcode 107126 en_US
dc.personcode 0000111870 en_US
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Mass spectrometry, Microbial proteomics, Outer membrane proteins, Two-dimensional gel electrophoresis, Two-dimensional liquid chromatography en_US
dc.staffid 107126 en_US

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