Optimization of Iron dependent cyanobacterial (Synechococcus, Cyanophyceae) bioreporters to measure iron bioavailability

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dc.contributor.author Hassler, Christel en_US
dc.contributor.author Twiss, R en_US
dc.contributor.author Mckay, R en_US
dc.contributor.author Bullerjahn, G en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:51Z
dc.date.available 2010-05-28T09:44:51Z
dc.date.issued 2006 en_US
dc.identifier 2008008817 en_US
dc.identifier.citation Hassler Christel et al. 2006, 'Optimization of Iron dependent cyanobacterial (Synechococcus, Cyanophyceae) bioreporters to measure iron bioavailability', Blackwell Publishing Ltd, vol. 42, no. 2, pp. 324-335. en_US
dc.identifier.issn 0022-3646 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8688
dc.description.abstract Complex chemistry and biological uptake pathways render iron bioavailability particularly difficult to assess in natural waters. Bioreporters are genetically modified organisms that are useful tools to directly sense the bioavailable fractions of solutes. In this study, three cyanobacterial bioreporters derived from Synechococcus PCC 7942 were examined for the purpose of optimizing the response to bioavailable Fe. Each bioreporter uses a Fe-regulated promoter ( isiAB, irpA and mapA), modulated by distinct mechanisms under Fe deficiency, fused to a bacterial luciferase ( luxAB). In order to provide a better understanding of the way natural conditions may affect the ability of the bioreporter to sense iron bioavailability, the effect of relevant environmental parameters on the response to iron was assessed. Optimal conditions (and limits of applicability) for the use of these bioreporters on the field were determined to be: a 12 h (12?24 h) exposure time, temperature of 15?C (15?C?22?C), photon flux density of 100 ?mol photons?m<sup>-2</sup>?s<sup>-1</sup> (37?200 lmol photons?m<sup>-2</sup>?s<sup>-1</sup>), initial biomass of 0.6?0.8 lg chlorophyll a (chl a)?L<sup>-1</sup> (0.3?1.5 lg chl a?L<sup>-1</sup>) or approximately 10<sup>5</sup> bioreporter cells?mL<sup>-1</sup>, high phosphate (10 lM), and low micronutrients (absent). en_US
dc.language en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1529-8817.2006.00203.x en_US
dc.title Optimization of Iron dependent cyanobacterial (Synechococcus, Cyanophyceae) bioreporters to measure iron bioavailability en_US
dc.parent Journal of Phycology en_US
dc.journal.volume 42 en_US
dc.journal.number 2 en_US
dc.publocation USA en_US
dc.identifier.startpage 324 en_US
dc.identifier.endpage 335 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 060700 en_US
dc.personcode 107808 en_US
dc.personcode 0000054119 en_US
dc.personcode 0000054120 en_US
dc.personcode 0000054121 en_US
dc.percentage 100 en_US
dc.classification.name Plant Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords bioavailability; bioreporter; cyanobacteria; iron; isiAB en_US
dc.staffid en_US

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