Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8

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dc.contributor.author Stokes, Harold en_US
dc.contributor.author Moore, R en_US
dc.contributor.author Krishnapillai, V en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:48Z
dc.date.available 2010-05-28T09:44:48Z
dc.date.issued 1981 en_US
dc.identifier 2008005152 en_US
dc.identifier.citation Stokes Harold, Moore R, and Krishnapillai V 1981, 'Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8', Academic Press, vol. 5, no. 2, pp. 202-212. en_US
dc.identifier.issn 0147-619X en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8679
dc.description.abstract A method of transductional complementation was developed in Pseudomonas aeruginosa to identify the cistrons involved in the conjugal transfer of the wide host range R plasmid R18. This used the P. aeruginosa bacteriophage E79tv-2 and has led to the identification of eight tra cistrons encoded by this plasmid. Plasmids mutant in six cistrons, traA, traB, traC, traD, traE, and traG were resistant to donor-specific phage (Dps-) while traF and traH mutant plasmids retained phage sensitivity. Some traB mutants were unable to inhibit the replication of phage G101 (Phi(G101)-) while some were also deficient in entry exclusion (Eex-). Two traB mutants which were also Eex- were suppressible by an amber suppressor. Three tra mutants selected directly as being Phi(G101)- were found to be also Dps-Eex- and mutant in traB. These data suggest a relationship between traB, Eex, and Phi(G101). In order to facilitate future genetic comparison of the tra genes of R18 and other wide host range plasmids and the role of the host in conjugation, R18 DNA was compared with that of RP4, by restriction enzyme fragment patterns and found to be identical en_US
dc.language en_US
dc.publisher Academic Press en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/0147-619X(81)90021-4 en_US
dc.title Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8 en_US
dc.parent Plasmid en_US
dc.journal.volume 5 en_US
dc.journal.number 2 en_US
dc.publocation United States en_US
dc.identifier.startpage 202 en_US
dc.identifier.endpage 212 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 105741 en_US
dc.personcode 0000050362 en_US
dc.personcode 0000047390 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US


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