The sedimentation equilibrium of heterogeneously associating systems and mixtures of non-interacting solutes: analysis without determination of molecular weight averages

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dc.contributor.author Nichol, L en_US
dc.contributor.author Jeffrey, P en_US
dc.contributor.author Milthorpe, Bruce en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:42Z
dc.date.available 2010-05-28T09:44:42Z
dc.date.issued 1976 en_US
dc.identifier 2008004095 en_US
dc.identifier.citation Nichol L, Jeffrey P, and Milthorpe Bruce 1976, 'The sedimentation equilibrium of heterogeneously associating systems and mixtures of non-interacting solutes: analysis without determination of molecular weight averages', Elsevier BV, vol. 4, no. 3, pp. 259-267. en_US
dc.identifier.issn 0301-4622 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8663
dc.description.abstract Sedimentation equilibrium is first considered of a system in which a ligand of any size binds to an acceptor at p sites, the experimental result, obtained with either interference or absorption optics, being a distribution of total solute concentration as a function of radial distance. Theory illustrated by a numerical example, is presented which shows that this distribution may be analysed to give the activity of the unbound ligand as a function of total weight concentration. It is shown that this information may be used together with conservation of mass equations written in terms of the initial mixing composition to evaluate the equilibrium constant(s) relevant to the system. Correlation with composition evaluation by use of absorption optics (when possible) is also discussed. The procedure does not involve solution of simultaneous equations which are sums of exponentials nor differentiation of experimental results to obtain apparent weight-average molecular weights. It is general in that it leads to the evaluation of the activity of the species characterized by the smallest M(l-Image ?) product and, accordingly, is shown to be useful in the analysis of non-interacting as well as of interacting systems. en_US
dc.language en_US
dc.publisher Elsevier BV en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/0301-4622(76)80073-7 en_US
dc.title The sedimentation equilibrium of heterogeneously associating systems and mixtures of non-interacting solutes: analysis without determination of molecular weight averages en_US
dc.parent Biophysical Chemistry en_US
dc.journal.volume 4 en_US
dc.journal.number 3 en_US
dc.publocation Netherlands en_US
dc.identifier.startpage 259 en_US
dc.identifier.endpage 267 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060100 en_US
dc.personcode 0000049736 en_US
dc.personcode 0000049735 en_US
dc.personcode 105631 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid 105631 en_US


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