Recovery of new integron classes from environmental DNA

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dc.contributor.author Nield, Blair en_US
dc.contributor.author Holmes, Andrew en_US
dc.contributor.author Gillings, Michael en_US
dc.contributor.author Recchia, Gavin en_US
dc.contributor.author Mabbutt, Bridget en_US
dc.contributor.author Nevalainen, Helena en_US
dc.contributor.author Stokes, Hatch en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:37Z
dc.date.available 2010-05-28T09:44:37Z
dc.date.issued 2001 en_US
dc.identifier 2008005394 en_US
dc.identifier.citation Nield Blair et al. 2001, 'Recovery of new integron classes from environmental DNA', Elsevier Inc, vol. 195, no. 1, pp. 59-65. en_US
dc.identifier.issn 0378-1097 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8649
dc.description.abstract Integrons are genetic elements known for their role in the acquisition and expression of genes conferring antibiotic resistance. Such acquisition is mediated by an integron-encoded integrase, which captures genes that are part of gene cassettes. To test whether integrons occur in environments with no known history of antibiotic exposure, PCR primers were designed to conserved regions of the integrase gene and the gene cassette recombination site. Amplicons generated from four environmental DNA samples contained features typical of the integrons found in antibiotic-resistant and pathogenic bacteria. The sequence diversity of the integrase genes in these clones was sufficient to classify them within three new classes of integron. Since they are derived from environments not associated with antibiotic use, integrons appear to be more prevalent in bacteria than previously observed. en_US
dc.language en_US
dc.publisher Elsevier Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/S0378-1097(00)00546-2 en_US
dc.title Recovery of new integron classes from environmental DNA en_US
dc.parent FEMS Microbiology Letters en_US
dc.journal.volume 195 en_US
dc.journal.number 1 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 59 en_US
dc.identifier.endpage 65 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 104673 en_US
dc.personcode 0000050528 en_US
dc.personcode 0000030144 en_US
dc.personcode 0000021502 en_US
dc.personcode 0000050529 en_US
dc.personcode 0000027049 en_US
dc.personcode 105741 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Integron ? Tyrosine recombinase ? Gene cassette ? Environmental DNA en_US
dc.staffid 105741 en_US


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