Quorum sensing regulation of adhesion in Serratia marcescens is surface dependant

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dc.contributor.author Labbate, Maurizio en_US
dc.contributor.author Zhu, Hua en_US
dc.contributor.author Thung, Leena en_US
dc.contributor.author Bandara, Rani en_US
dc.contributor.author Learsen, Martin en_US
dc.contributor.author Willcox, Mark en_US
dc.contributor.author Givskov, Michael en_US
dc.contributor.author Rice, Scott en_US
dc.contributor.author Kjelleberg, Staffan en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:36Z
dc.date.available 2010-05-28T09:44:36Z
dc.date.issued 2007 en_US
dc.identifier 2008003898 en_US
dc.identifier.citation Labbate Maurizio et al. 2007, 'Quorum sensing regulation of adhesion in Serratia marcescens is surface dependant', Amer Soc Microbiology, vol. 189, pp. 2702-2711. en_US
dc.identifier.issn 0021-9193 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8648
dc.description.abstract Serratia marcescens is an opportunistic pathogen and a major cause of ocular infections. In previous studies of S. marcescens MG1, we showed that biofilm maturation and sloughing were regulated by N-acyl homoserine lactone (AHL)-based quorum sensing (QS). Because of the importance of adhesion in initiating biofilm formation and infection, the primary goal of this study was to determine whether QS is important in adhesion to both abiotic and biotic surfaces, as assessed by determining the degree of attachment to hydrophilic tissue culture plates and human corneal epithelial (HCE) cells. Our results demonstrate that while adhesion to the abiotic surface was AHL regulated, adhesion to the HCE cell biotic surface was not. Type I fimbriae were identified as the critical adhesin for non-QS-mediated attachment to the biotic HCE cell surface but played no role in adhesion to the abiotic surface. While we were not able to identify a single QS-regulated adhesin essential for attachment to the abiotic surface, four AHL-regulated genes involved in adhesion to the abiotic surface were identified. Interestingly, two of these genes, bsmA and bsmB, were also shown to be involved in adhesion to the biotic surface in a non-QS-controlled fashion. Therefore, the expression of these two genes appears to be cocontrolled by regulators other than the QS system for mediation of attachment to HCE cells. We also found that QS in S. marcescens regulates other potential cell surface adhesins, including exopolysaccharide and the outer membrane protein OmpX. We concluded that S. marcescens MG1 utilizes different regulatory systems and adhesins in attachment to biotic and abiotic surfaces and that QS is a main regulatory pathway in adhesion to an abiotic surface but not in adhesion to a biotic surface. en_US
dc.language en_US
dc.publisher Amer Soc Microbiology en_US
dc.relation.isbasedon http://dx.doi.org/10.1128/JB.01582-06 en_US
dc.title Quorum sensing regulation of adhesion in Serratia marcescens is surface dependant en_US
dc.parent Journal Of Bacteriology en_US
dc.journal.volume 189 en_US
dc.journal.number en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 2702 en_US
dc.identifier.endpage 2711 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 106011 en_US
dc.personcode 0000050238 en_US
dc.personcode 0000050240 en_US
dc.personcode 0000050241 en_US
dc.personcode 0000050242 en_US
dc.personcode 0000034482 en_US
dc.personcode 0000050243 en_US
dc.personcode 0000050237 en_US
dc.personcode 0000027051 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US

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