An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection

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dc.contributor.author Mcarthur, Jason en_US
dc.contributor.author West, Nicholas en_US
dc.contributor.author Cole, J en_US
dc.contributor.author Jungnitz, Heidrun en_US
dc.contributor.author Guzman, Carlos en_US
dc.contributor.author Chin, James en_US
dc.contributor.author Lehrbach, Phillip en_US
dc.contributor.author Djordjevic, Steven en_US
dc.contributor.author Walker, Mark en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:44:32Z
dc.date.available 2010-05-28T09:44:32Z
dc.date.issued 2003 en_US
dc.identifier 2008007105 en_US
dc.identifier.citation Mcarthur Jason et al. 2003, 'An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection', Wiley-Blackwell Publishing Ltd., vol. 221, pp. 7-16. en_US
dc.identifier.issn 0378-1097 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8634
dc.description.abstract We have constructed an aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica, harbouring mutations in aroA and trpE to investigate the use of such a strain as a live-attenuated vaccine. B. bronchiseptica aroA trpE was unable to grow in minimal medium without aromatic supplementation. Compared to the parental wild-type strain, the mutant displayed significantly reduced abilities to invade and survive within the mouse macrophage-like cell line J774A.1 in vitro and in the murine respiratory tract following experimental intranasal infection. Mice vaccinated with B. bronchiseptica aroA trpE displayed significant dose-dependent increases in B. bronchiseptica-specific antibody responses, and exhibited increases in the number of B. bronchiseptica-reactive spleen cells in lymphoproliferation assays. Immunised animals were protected against lung colonisation after challenge with the wild-type parental strain. With such a broad host range displayed by B. bronchiseptica, the attenuated strain constructed in this study may not only be used for the prevention of B. bronchiseptica-associated disease, but also for the potential delivery of heterologous antigen. en_US
dc.language en_US
dc.publisher Wiley-Blackwell Publishing Ltd. en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/S0378-1097(03)00162-9 en_US
dc.title An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection en_US
dc.parent FEMS Microbiology Letters en_US
dc.journal.volume 221 en_US
dc.journal.number en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 7 en_US
dc.identifier.endpage 16 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000052368 en_US
dc.personcode 0000052369 en_US
dc.personcode 0000052325 en_US
dc.personcode 0000052370 en_US
dc.personcode 0000052371 en_US
dc.personcode 0000018318 en_US
dc.personcode X000153 en_US
dc.personcode 107126 en_US
dc.personcode 0000035449 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US


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