Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water

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Show simple item record Aridgides, Laura en_US Doblin, Martina en_US Berke, T en_US Dobbs, Fred en_US Matson, David en_US Drake, Lisa en_US
dc.contributor.editor en_US 2010-05-28T09:44:30Z 2010-05-28T09:44:30Z 2004 en_US
dc.identifier 2006007049 en_US
dc.identifier.citation Aridgides Laura et al. 2004, 'Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water', Elsevier Ltd, vol. 48, no. 11-12, pp. 1096-1101. en_US
dc.identifier.issn 0025-326X en_US
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract There is enormous potential for global transfer of microorganisms, including pathogens, in ships' ballast water. We contend that a major advancement in the study of ballast-water microorganisms in particular, and of aquatic pathogens in general, will be expedited sample analysis, such as provided by the elegant technology of DNA microarrays. In order to use DNA microarrays, however, one must establish the appropriate conditions to bind target sequences in samples to multiple probes on the microarrays. We conducted proof-of-concept experiments to optimize simultaneous detection of multiple microorganisms using polymerase chain reaction (PCR) and Southern hybridization. We chose three target organisms, all potentially found in ballast water: a calicivirus, the bacterium Vibrio cholerae, and the photosynthetic protist Aureococcus anophagefferens. Here, we show simultaneous detection of multiple pathogens is possible, a result supporting the promising future use of microarrays for simultaneous detection of pathogens in ballast water. en_US
dc.language en_US
dc.publisher Elsevier Ltd en_US
dc.relation.isbasedon en_US
dc.title Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water en_US
dc.parent Marine Pollution Bulletin en_US
dc.journal.volume 48 en_US
dc.journal.number 11-12 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 1096 en_US
dc.identifier.endpage 1101 en_US SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 060205 en_US
dc.personcode 0000046202 en_US
dc.personcode 996445 en_US
dc.personcode 0000046203 en_US
dc.personcode 0000027780 en_US
dc.personcode 0000046204 en_US
dc.personcode 0000026155 en_US
dc.percentage 100 en_US Marine and Estuarine Ecology (incl. Marine Ichthyology) en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom 2.007 en_US en_US
dc.location.activity en_US

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