Discriminating between intra- and extracellular metals using chemical extractions: an update on the case of iron

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dc.contributor.author Hassler, Christel en_US
dc.contributor.author Schoemann, V en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:43:51Z
dc.date.available 2010-05-28T09:43:51Z
dc.date.issued 2009 en_US
dc.identifier 2008008824 en_US
dc.identifier.citation Hassler Christel and Schoemann V 2009, 'Discriminating between intra- and extracellular metals using chemical extractions: an update on the case of iron', American Society of Limnology and Oceanography Inc., vol. 7, no. NA, pp. 479-489. en_US
dc.identifier.issn 1541-5856 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8539
dc.description.abstract Iron influences the climate system by limiting primary productivity. It is therefore essential to accurately measure the iron fraction associated with phytoplankton in aquatic systems. A washing procedure using EDTA, being efficient for numerous trace metals, is not strong enough to remove iron adsorbed to the surface of microorganisms. Stronger washing solutions are used for iron, but these have only been assessed for a marine diatom. This study assesses the applicability of the oxalate washing procedure for both fresh- and seawater aquatic systems. We assessed iron solubilization as a result of oxalate washing in both synthetic and natural freshwater and seawater, and we tested it on several model phytoplankton and natural assemblages from Lake Champlain, the Southern Ocean, and the Derwent River estuary. We report the effects of the oxalate solution contact time, concentration, and amendment. Our study shows that 20-min washing provides an efficient measurement of the intracellular phytoplanktonic pool of iron in both freshwater and seawater. The direct amendment of oxalate in the experimental solution presents many advantages that are critical for the measurement of size-fractionated particulate iron. These include fine control of bioaccumulation termination, a significant gain in time, and homogeneity of the washing treatment. en_US
dc.language en_US
dc.publisher American Society of Limnology and Oceanography Inc. en_US
dc.relation.isbasedon http://dx.doi.org/10.4319/lom.2009.7.479 en_US
dc.title Discriminating between intra- and extracellular metals using chemical extractions: an update on the case of iron en_US
dc.parent Limnology and Oceanography: Methods en_US
dc.journal.volume 7 en_US
dc.publocation Waco Texas, USA en_US
dc.identifier.startpage 479 en_US
dc.identifier.endpage 489 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060200 en_US
dc.personcode 107808 en_US
dc.personcode 0000054130 en_US
dc.percentage 100 en_US
dc.classification.name Oceanography en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US

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