Distinguishing intra- from extracellular metals: chemical extraction techniques

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dc.contributor.author Hassler, Christel en_US
dc.contributor.author Slaveykova, V en_US
dc.contributor.author Wilkinson, K en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:43:48Z
dc.date.available 2010-05-28T09:43:48Z
dc.date.issued 2004 en_US
dc.identifier 2008008815 en_US
dc.identifier.citation Hassler Christel, Slaveykova V, and Wilkinson K 2004, 'Distinguishing intra- from extracellular metals: chemical extraction techniques', American Society of Limnology and Oceanography Inc., vol. 2, no. 1, pp. 237-247. en_US
dc.identifier.issn 1541-5856 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8531
dc.description.abstract Several washing agents (EDTA, CDTA, NTA, 8 HQS, Ti-EDTA-citrate, Ca2+, and H+) were tested for their ability to extract adsorbed metal from biological surfaces to distinguish between internalized and total cellular metal (Pb, Ni, Cd, Zn, and Cu) for a green alga, Chlorella kesslerii. Although a single extraction protocol cannot be provided that would be acceptable for all biological species and metals, several guidelines are proposed that should facilitate the interpretation of metal bioaccumulation data. It was shown that for the studied metals, a thermodynamic explanation was generally sufficient to explain the metal extraction results. At pH 6.0, a solution of 5 ? 10?3 M ethylenediaminetetraacetic acid (EDTA) was an acceptable washing agent in most cases, whereas Ca or proton exchange solutions did not appear to quantitatively remove adsorbed metals. Conditions of ligand excess could be attained with relatively small concentrations of added ligand, effectively minimizing potential damage to the organism, including modifications in membrane permeability. Potential deleterious effects of the wash agents were verified. For example, a Ti-EDTA-citrate or an EDTA wash at high concentrations resulted in perturbations of algal membrane permeability. In spite of potential difficulties interpreting extraction data, the usefulness of the extraction protocols was clearly demonstrated for several organisms. en_US
dc.language en_US
dc.publisher American Society of Limnology and Oceanography Inc. en_US
dc.title Distinguishing intra- from extracellular metals: chemical extraction techniques en_US
dc.parent Limnology and Oceanography: Methods en_US
dc.journal.volume 2 en_US
dc.journal.number 1 en_US
dc.publocation Waco Texas, USA en_US
dc.identifier.startpage 237 en_US
dc.identifier.endpage 247 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 040502 en_US
dc.personcode 107808 en_US
dc.personcode 0000054116 en_US
dc.personcode 0000054117 en_US
dc.percentage 100 en_US
dc.classification.name Chemical Oceanography en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US

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