A unique thioredoxin of the parasitic nematode Haemonchus contortus with glutaredoxin activity

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dc.contributor.author Sotirchos, Irene en_US
dc.contributor.author Hudson, Amanda en_US
dc.contributor.author Ellis, John en_US
dc.contributor.author Davey, Mary en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:43:21Z
dc.date.available 2010-05-28T09:43:21Z
dc.date.issued 2009 en_US
dc.identifier 2008007498 en_US
dc.identifier.citation Sotirchos Irene et al. 2009, 'A unique thioredoxin of the parasitic nematode Haemonchus contortus with glutaredoxin activity', Elsevier, vol. 46, pp. 579-585. en_US
dc.identifier.issn 0891-5849 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/8462
dc.description.abstract en_US
dc.description.abstract The dependency of parasites on the cellular redox systems has led to their investigation as novel drug targets. Defence against oxidative damage is through the thioredoxin and glutathione systems. The classic thioredoxin is identified by the active site Cys-Gly-Pro-Cys (CGPC). Here we describe the identification of a unique thioredoxin in the parasitic nematode, Haemonchus contortus. This thioredoxin-related protein, termed HcTrx5, has an arginine in its active site (Cys-Arg-Ser-Cys; CRSC) that is not found in any other organism. Recombinant HcTrx5 was able to reduce the disulfide bond in insulin, and be regenerated by mammalian thioredoxin reductase with a Km 2.19 ? 1.5 ?M, similar to the classic thioredoxins. However, it was also able to reduce insulin when glutathione and glutathione reductase replaced the thioredoxin reductase. When coupled with H. contortus peroxiredoxin, HcTrx5 was active using either the thioredoxin reductase or the glutathione and glutathione reductase. HcTrx5 is expressed through the life cycle, with highest expression in the adult stage. The unique activity of this thioredoxin makes it a potential drug target for the control of this parasite. en_US
dc.language en_US
dc.publisher Elsevier en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.freeradbiomed.2008.11.009 en_US
dc.title A unique thioredoxin of the parasitic nematode Haemonchus contortus with glutaredoxin activity en_US
dc.parent Free Radical Biology and Medicine en_US
dc.journal.volume 46 en_US
dc.journal.number en_US
dc.journal.number 5 en_US
dc.publocation Switzerland en_US
dc.identifier.startpage 579 en_US
dc.identifier.endpage 585 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 070700 en_US
dc.personcode 99068203 en_US
dc.personcode 030905 en_US
dc.personcode 910945 en_US
dc.personcode 880134 en_US
dc.percentage 100 en_US
dc.classification.name Veterinary Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US
dc.description.keywords Haemonchus contortus; Nematode; Thioredoxin; Glutaredoxin; Functional characterisation; Drug target en_US
dc.staffid en_US
dc.staffid 880134 en_US

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