Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models

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dc.contributor.author Hare, Dominic en_US
dc.contributor.author Reedy, Brian en_US
dc.contributor.author Grimm, Rudolf en_US
dc.contributor.author Wilkins, Simon en_US
dc.contributor.author Volitakis, Irene en_US
dc.contributor.author George, Jessica en_US
dc.contributor.author Cherny, Robert en_US
dc.contributor.author Bush, Ashley en_US
dc.contributor.author Finkelstein, David en_US
dc.contributor.author Doble, Philip en_US
dc.contributor.editor en_US
dc.date.accessioned 2010-05-28T09:43:06Z
dc.date.available 2010-05-28T09:43:06Z
dc.date.issued 2009 en_US
dc.identifier 2008002393 en_US
dc.identifier.citation Hare Dominic et al. 2009, 'Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models', Royal Society of Chemistry, vol. 1, no. 1, pp. 53-58. en_US
dc.identifier.issn 1756-5901 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/8417
dc.description.abstract This study demonstrates the application of quantitative elemental bio-imaging for the determination of the distribution Cu, Mn, Fe and Zn in Parkinsonism mouse model brains. Elevated concentrations of these metals within the substantia nigra (SN) are suspected to play a role on the development of Parkinsons disease. Elemental bio-imaging employs laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to construct images of trace element distribution. Quantitative data was produced by ablating the standard tissue sections and recording the mean signal intensity calibrated against multi level matrix matched tissue standards. The concentrations of Fe within the substantia nigra of the lesioned animals increased significantly when compared against control animals. Furthermore, the data was compared against solution nebulisation ICP-MS in which the whole substantia nigra was excised. The trends were the same for both methods; however the elemental bio-imaging method returned significantly higher concentrations. This was caused by dilution from inclusion of surrounding tissue of the SN during the excision procedure. en_US
dc.language en_US
dc.publisher Royal Society of Chemistry en_US
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon http://dx.doi.org/10.1039/b816188g en_US
dc.title Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models en_US
dc.parent metallomics en_US
dc.journal.volume 1 en_US
dc.journal.number 1 en_US
dc.publocation London, UK en_US
dc.identifier.startpage 53 en_US
dc.identifier.endpage 58 en_US
dc.cauo.name SCI.Chemistry and Forensic Sciences en_US
dc.conference Verified OK en_US
dc.for 039902 en_US
dc.personcode 995243 en_US
dc.personcode 000263 en_US
dc.personcode 0000048940 en_US
dc.personcode 0000053929 en_US
dc.personcode 0000053930 en_US
dc.personcode 0000053931 en_US
dc.personcode 0000050014 en_US
dc.personcode 0000046487 en_US
dc.personcode 0000046486 en_US
dc.personcode 010494 en_US
dc.percentage 100 en_US
dc.classification.name Forensic Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid 010494 en_US

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