Development of a Cryptosporidium oocyst assay using an automated fiber optic-based biosensor

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dc.contributor.author Kramer, Marianne en_US
dc.contributor.author Vesey, Graham en_US
dc.contributor.author Look, Natasha en_US
dc.contributor.author Herbert, Ben en_US
dc.contributor.author Simpson-Stroot, Joyce en_US
dc.contributor.author Lim, Daniel en_US
dc.date.accessioned 2009-12-21T03:53:18Z
dc.date.available 2009-12-21T03:53:18Z
dc.date.issued 2007 en_US
dc.identifier 2007002461 en_US
dc.identifier.citation Kramer Marianne et al. 2007, 'Development of a Cryptosporidium oocyst assay using an automated fiber optic-based biosensor', Biomed Central, vol. 1, no. 3, pp. 1-11. en_US
dc.identifier.issn 1754-1611 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/5859
dc.description.abstract An intestinal protozoan parasite, Cryptosporidium parvum, is a major cause of waterborne gastrointestinal disease worldwide. Detection of Cryptosporidium oocysts in potable water is a high priority for the water treatment industry to reduce potential outbreaks among the consumer populace. Anti-Cryptosporidium oocyst polyclonal and monoclonal antibodies were tested as capture and detection reagents for use in a fiber optic biosensor assay for the detection of Cryptosporidium oocysts. Antibodies were validated using enzyme-linked immunosorbent assays, flow cytometry, Western blotting and fluorescent microscopy. Oocysts could be detected at a concentration of 105 oocysts/ml when the polyclonal antibodies were used as the capture and detection reagents. When oocysts were boiled prior to detection, a ten-fold increase in sensitivity was achieved using the polyclonal antibody. Western blotting and immunofluorescence revealed that both the monoclonal and polyclonal antibodies recognize a large (>300 kDa) molecular weight mucin-like antigen present on the surface of the oocyst wall. The polyclonal antibody also reacted with a small (105 kDa) molecular weight antigen that was present in boiled samples of oocysts. Preliminary steps to design an in-line biosensor assay system have shown that oocysts would have to be concentrated from water samples and heat treated to allow detection by a biosensor assay. en_US
dc.publisher Biomed Central en_US
dc.title Development of a Cryptosporidium oocyst assay using an automated fiber optic-based biosensor en_US
dc.parent Journal of Biological Engineering en_US
dc.journal.volume 1 en_US
dc.journal.number 3 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 1 en_US
dc.identifier.endpage 11 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060109 en_US
dc.personcode 0000042933 en_US
dc.personcode 104101 en_US
dc.personcode 0000042934 en_US
dc.personcode 997815 en_US
dc.personcode 0000042936 en_US
dc.personcode 0000042937 en_US
dc.percentage 100 en_US
dc.classification.name Proteomics and Intermolecular Interactions (excl. Medical Proteomics) en_US
dc.classification.type FOR-08 en_US


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