Biosynthetic incorporation of oxidised amino acids into proteins and their cellular proteolysis

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dc.contributor.author Rodgers, K. J en_US
dc.contributor.author Wang, H. en_US
dc.contributor.author Fu, S. en_US
dc.contributor.author Dean, R. T. en_US
dc.date.accessioned 2009-06-26T04:10:51Z
dc.date.available 2009-06-26T04:10:51Z
dc.date.issued 2002 en_US
dc.identifier 2008001016 en_US
dc.identifier.citation Rodgers, K. et al. 2002 'Biosynthetic incorporation of oxidised amino acids into proteins and their cellular proteolysis', Free Radical Biology and Medicine, vol. 32, no. 8, pp. 766-775. en_US
dc.identifier.issn 0891-5849 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/555
dc.description.abstract We demonstrate that oxidized amino acids can be incorporated into proteins by protein synthesis. The level of incorporation into protein was dependent on the concentration of oxidized amino acid supplied to the cells. At low levels of incorporation, the oxidized amino acids examined increased the degradation rate of the cell proteins. Degradation of certain proteins containing high levels of DOPA (but not ortho or meta tyrosine) was decreased to below the basal degradation rates suggesting that DOPA may contribute to proteins becoming resistant to proteolysis. Changes in the degradation rates of the oxidized amino acid-containing proteins was shown to have no impact on the degradation rates of native proteins, indicating that the activity of the degradative machinery was not affected. We demonstrate that oxidized proteins are selectively degraded by the proteasomes and provide evidence to suggest that the proteasomes and the endosomal-lysosomal systems may act in sequence as well as in parallel. The incorporation approach, unlike cell studies in which an exogenous oxidant is used, allows the degradation rates of the oxidatively modified proteins to be selectively measured, offering a greater sensitivity as well as greatly reducing toxicity to the cell and avoiding oxidative modification of other cell components. en_US
dc.publisher Pergamon en_US
dc.relation.isbasedon http//dx.doi.org/10.1016/S08915849(02)007682 en_US
dc.title Biosynthetic incorporation of oxidised amino acids into proteins and their cellular proteolysis en_US
dc.parent Free Radical Biology and Medicine en_US
dc.journal.volume 32 en_US
dc.journal.number 8 en_US
dc.publocation Oxford UK en_US
dc.identifier.startpage 766 en_US
dc.identifier.endpage 775 en_US
dc.cauo.name Chemistry Materials and Forensic Sciences en_US


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