Rapid assessment of endocrine disruption: vitellogenin (Vtg) expression in male estuarine toadfish (Tetratenos glaber Tetraodontiformes)

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dc.contributor.author Booth, David en_US
dc.contributor.author Skene, Caroline en_US
dc.date.accessioned 2009-12-21T02:34:38Z
dc.date.available 2009-12-21T02:34:38Z
dc.date.issued 2006 en_US
dc.identifier 2006005692 en_US
dc.identifier.citation Booth David and Skene Caroline 2006, 'Rapid assessment of endocrine disruption: vitellogenin (Vtg) expression in male estuarine toadfish (Tetratenos glaber Tetraodontiformes)', Australasian Society for Ecotoxicology, vol. 12, no. 1, pp. 3-8. en_US
dc.identifier.issn 1323-3475 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4752
dc.description.abstract Increased contamination of waterways has lead to many impacts on organisms, including effects on reproduction. A suite of endocrine-disruptive chemicals (DECs) has been shown to mimic sex hormones in vertebrates and their presence is an important bioindicator of environmental degradation. We examined expression of vitellogenin (Vtg, a female yolk protein) in male toadfish (Tetratenos glaber), as an indicator of EDC presence in estuaries around Sydney, Australia. First we demonstrated induction of Vtg in males from unpolluted estuarine sites through injection of 17beta-oestradiol. Second, serum of fish from polluted and unpolluted estuaries was collected and examined by reducing-polyacryamide gel electrophoresis (SDS-PAGE). While females from polluted (downstream from sewage treatment plants, and subject to urban runoff) and less polluted sites all expressed Vtg in blood serum, males from less polluted sites showed little or no evidence of Vtg expression. however, most males from heavilty polluted sites showed moderate to high levels of vtg expression indicating that EDCs were present and affecting normal endocrine function in males. We suggest that simple biochemical examinations of EDS effects, such as vtg induction in males, are useful rapid assessment methods wich can provide evidence upon which further, more detailed studies may be undertaken. en_US
dc.publisher Australasian Society for Ecotoxicology en_US
dc.relation.isbasedon en_US
dc.title Rapid assessment of endocrine disruption: vitellogenin (Vtg) expression in male estuarine toadfish (Tetratenos glaber Tetraodontiformes) en_US
dc.parent Australasian Journal of Ecotoxicology en_US
dc.journal.volume 12 en_US
dc.journal.number 1 en_US
dc.publocation Cammeray NSW, Australia en_US
dc.identifier.startpage 3 en_US
dc.identifier.endpage 8 en_US
dc.cauo.name DVCRch.Institute for Water & Environmental Resource Mgmnt en_US
dc.conference Verified OK en_US
dc.for 039900 en_US
dc.personcode 940138 en_US
dc.personcode 0000022559 en_US
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.description.keywords edocrine-disruptive compounds, estarine fishes, sewage effluent, toadfish (Tetratenos glaber), vitellogenin en_US


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