Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation

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dc.contributor.author Peters, Phoebe en_US
dc.contributor.author Thoni, C en_US
dc.contributor.author Harry, Liz en_US
dc.date.accessioned 2009-12-21T02:32:58Z
dc.date.available 2009-12-21T02:32:58Z
dc.date.issued 2006 en_US
dc.identifier 2006004124 en_US
dc.identifier.citation Peters Phoebe, Thoni C, and Harry Elizabeth 2006, 'Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation', B I O S Scientific Publishers Ltd, vol. 81, no. 2-3, pp. 105-106. en_US
dc.identifier.issn 1052-0295 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4352
dc.description.abstract A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when us en_US
dc.publisher B I O S Scientific Publishers Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1080/10520290600869355 en_US
dc.title Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation en_US
dc.parent Biotechnic and Histochemistry en_US
dc.journal.volume 81 en_US
dc.journal.number 2-3 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 105 en_US
dc.identifier.endpage 106 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110800 en_US
dc.personcode 996413 en_US
dc.personcode 0000027894 en_US
dc.personcode 995003 en_US
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Alexa 488; bacteria; immunofluorescence microscopy; photobleaching; 2-photon excitation en_US
dc.staffid 995003 en_US


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