Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp

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Show simple item record Villavedra Sierra, Margarita en_US Lemke, Susan en_US To, Joyce en_US Broady, Kevin en_US Wallach, Michael en_US Raison, Robert en_US 2009-12-21T02:30:39Z 2009-12-21T02:30:39Z 2007 en_US
dc.identifier 2006009642 en_US
dc.identifier.citation Villavedra Sierra Margarita et al. 2007, 'Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp.', Blackwell Publishing Ltd, vol. 30, pp. 191-199. en_US
dc.identifier.issn 0140-7775 en_US
dc.identifier.other C1 en_US
dc.description.abstract Amoebic gill disease, the main disease of concern to the salmon industry is Tasmania is caused by the amoeba Neoparamoeba spp. Experimental infection can onlybe induced by exposure to wild-type (WT) oarasites isolated from the gills of infected fish, as cultured amoeba are non-infective. To characterise the surface antigens of WT parasites, we produced monoclonal antibodies (mAbs) using subtractive immunization. Mice inoculated with non-infective parasites were treated with cyclophosphamide, to deplete reactive lumphocytes, and then immunized with different antigen preparations from infective parasites. When whole parasites were used for boosting the percentage of WT unique mAbs was very high (86%) as was the percentage of mAbs specific for carbohydrate pritopes (89%). When degloycosylated membranes were used, the numbers of mAbs spefic for non-carbohydrate spitopes id not increase, but the total number of WT unique mAbs was reduced (86-40%). Using an untreated membrane preparation, the total number of mAbs to surface molecules was very high, but all recognized carbohydrate epitopes. The total number of MAbs recognising carbohydrate epitopes on the surface of the WT parasites was 97%, suggesting that the dominant epitopes on the surface molecules unique to WT parasites are carbohydrate in nature. en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isbasedon en_US
dc.title Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp en_US
dc.parent Journal Of Fish Diseases en_US
dc.journal.volume 30 en_US
dc.journal.number en_US
dc.journal.number 4 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 191 en_US
dc.identifier.endpage 199 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 070400 en_US
dc.personcode 021127 en_US
dc.personcode 00015723 en_US
dc.personcode 98034263 en_US
dc.personcode 850065 en_US
dc.personcode 021128 en_US
dc.personcode 890404 en_US
dc.percentage 100 en_US Fisheries Sciences en_US
dc.classification.type FOR-08 en_US
dc.description.keywords amoebic gill disease; Atlantic salmon; carbohydrate epitopes, monoclonal antibodies, Neoparamoeba, substrative immunization en_US
dc.staffid 890404 en_US

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