Changes in gene expression associated with stable drug and radiation resistance in small cell lung cancer cells are similar to those caused by a single X-ray dose

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dc.contributor.author Henness, Sheridan en_US
dc.contributor.author Davey, Mary en_US
dc.contributor.author Harvie, Rozelle en_US
dc.contributor.author Banyer, Joanne en_US
dc.contributor.author Wasinger, Valerie en_US
dc.contributor.author Corthals, Garry en_US
dc.contributor.author Davey, Ross en_US
dc.date.accessioned 2009-12-21T02:30:18Z
dc.date.available 2009-12-21T02:30:18Z
dc.date.issued 2004 en_US
dc.identifier 2004000104 en_US
dc.identifier.citation Henness Sheridan et al. 2004, 'Changes in gene expression associated with stable drug and radiation resistance in small cell lung cancer cells are similar to those caused by a single X-ray dose', Radiation Research Soc, vol. 161, pp. 495-503. en_US
dc.identifier.issn 0033-7587 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3825
dc.description.abstract Small cell lung cancer (SCLC) initially responds well to chemotherapy and fractionated radiotherapy, but resistance to these treatments eventually develops in the vast majority of cases. To understand how resistance develops in the H69 SCLC cell line, we compared the changes in gene expression associated with 37.5 Gy fractionated X-ray treatment that produced the stable radiation- and drug-resistant H69/R38 cell subline to the changes associated with a single 4- or 8-Gy X-ray treatment. Gene expression was determined by suppression subtractive hybridization combined with Northern blot analysis and two-dimensional (2D) protein electrophoresis. Stable radiation and drug resistance was associated with coordinate changes in the expression of genes of the cytoskeleton, protein synthesis, cell cycle, redox/stress and metabolic pathways. The pattern of these changes was remarkably similar to the changes seen 24 h after a single X-ray treatment of the H69 cells but differed from the changes in expression associated with a single X-ray treatment of the resistant H69/ R38 cells. Stable radiation and drug resistance may be caused by the constitutive expression of those genes transiently expressed by sensitive cells in response to a single X-ray dose. The repeated treatments received during fractionated irradiation may promote the change from a transient to a constitutive pattern of gene expression. en_US
dc.publisher Radiation Research Soc en_US
dc.relation.isbasedon http://dx.doi.org/10.1667/RR3165 en_US
dc.title Changes in gene expression associated with stable drug and radiation resistance in small cell lung cancer cells are similar to those caused by a single X-ray dose en_US
dc.parent Radiation Research en_US
dc.journal.volume 161 en_US
dc.journal.number en_US
dc.publocation Oak Brook en_US
dc.identifier.startpage 495 en_US
dc.identifier.endpage 503 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060199 en_US
dc.personcode 0000021893 en_US
dc.personcode 880134 en_US
dc.personcode 0000021894 en_US
dc.personcode 0000021895 en_US
dc.personcode 0000021896 en_US
dc.personcode 0000021897 en_US
dc.personcode 100152 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology not elsewhere classified en_US
dc.classification.type FOR-08 en_US
dc.description.keywords NA en_US
dc.staffid 100152 en_US


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