Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony

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Show simple item record Ulstrup, Karin Elizabeth en_US Ralph, Peter en_US Van Oppen, Madeleine en_US Kuhl, Michael en_US 2009-12-21T02:30:16Z 2009-12-21T02:30:16Z 2007 en_US
dc.identifier 2007002653 en_US
dc.identifier.citation Ulstrup Karin Elizabeth et al. 2007, 'Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony', Springer, vol. 153, no. 2, pp. 225-234. en_US
dc.identifier.issn 0025-3162 en_US
dc.identifier.other C1 en_US
dc.description.abstract Corals harbouring genetically mixed communities of endosymbiotic algae (Symbiodinium) often show distribution patterns in accordance with differences in light climate across an individual colony. However, the physiology of these genetically characterised communities is not well understood. Single stranded conformation polymorphism (SSCP) and real time quantitative polymerase chain reaction (qPCR) analyses were used to examine the genetic diversity of the Symbiodinium community in hospite across an individual colony of Acropora valida at the spatial scale of single polyps. The physiological characteristics of the polyps were examined prior to sampling with a combined O2 microelectrode with a fibre-optic microprobe (combined sensor diameter 50-100 I?m) enabling simultaneous measurements of O2 concentration, gross photosynthesis rate and photosystem II (PSII) quantum yield at the coral surface as a function of increasing irradiances. Both sun- and shade-adapted polyps were found to harbour either Symbiodinium clade C types alone or clades A and C simultaneously. Polyps were grouped in two categories according to (1) their orientation towardps light, or (2) their symbiont community composition. Physiological differences were not detected between sun- and shade-adapted polyps, but O2 concentration at 1,100 I?mol photons m-2 s-1 was higher in polyps that harboured both clades A and C symbionts than in polyps that harboured clade C only. These results suggest that the acclimatisation of zooxanthellae of individual polyps of an A. valida colony to ambient light levels may not be the only determinant of the photosynthetic capacity of zooxanthellae. Here, we found that photosynthetic capacity is also likely to have a strong genetic basis and differs between genetically distinct Symbiodinium types. en_US
dc.publisher Springer en_US
dc.relation.isbasedon en_US
dc.title Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony en_US
dc.parent Marine Biology: international journal on life in oceans and coastal waters en_US
dc.journal.volume 153 en_US
dc.journal.number 2 en_US
dc.publocation Germany en_US
dc.identifier.startpage 225 en_US
dc.identifier.endpage 234 en_US SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 060200 en_US
dc.personcode 10018923 en_US
dc.personcode 890085 en_US
dc.personcode 0000017308 en_US
dc.personcode 107129 en_US
dc.percentage 100 en_US Ecology en_US
dc.classification.type FOR-08 en_US
dc.description.keywords acclimation; adaptation; alga; community composition; coral; endosymbiont; genetic analysis; irradiance; photosynthesis; physiology; polymerase chain reaction; polyp; real time; zooxanthella; Acropora valida; algae; Anthozoa; Symbiodinium en_US
dc.staffid 107129 en_US

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