Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design

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dc.contributor.author Mulholland, M. en_US
dc.contributor.author Prochazka, S. en_US
dc.contributor.author Lloyd-Jones, A. en_US
dc.date.accessioned 2009-12-21T02:28:54Z
dc.date.available 2009-12-21T02:28:54Z
dc.date.issued 2003 en_US
dc.identifier 2003000445 en_US
dc.identifier.citation Prochazka, S., Mulholland, M., and Lloyd-Jones, A. 2003 'Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design', Journal Of Pharmaceutical And Biomedical Analysis, vol. 31, no. 1, pp. 977-993. en_US
dc.identifier.issn 0731-7085 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3573
dc.description.abstract The separation by reverse polarity capillary zone electrophoresis of the therapeutically developed sodium salt of Pentosan Polysulfate was optimised through the analysis of response surface methodologies, modeled using a central composite design. The optimisation investigated injection pressure, injection time and voltage and the effect of the conditions on retention times, peak areas, separation efficiency and the method sensitivity. The overall goal was to develop the most sensitive results with no decrease in separation efficiency. The following results were obtained: (1) retention times generally decreased as injection pressure, injection time and voltage increased, injection time having the least effect; (2) as expected peak areas increased as injection pressure and injection time increased but decreased as voltage increased; (3) separation efficiencies generally increased as injection pressure and injection time decreased, with voltage having almost no effect. For the optimum condition, the sample was introduced at the inlet vial at the cathode hydrodynamically, at optimal setting of 44 s at 35 mbar. The optimal voltage was -20 kV. In comparison with other methods, the optimum showed increased sensitivity, resolution and separation efficiency. Repeatability studies were performed on the optimum parameter conditions. Relative standard deviation values obtained were between 0.9 and 5.4%. en_US
dc.publisher Elsevier en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/S0960-0779(03)00067-5 en_US
dc.title Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design en_US
dc.parent Journal Of Pharmaceutical And Biomedical Analysis en_US
dc.journal.volume 31 en_US
dc.journal.number 1 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 977 en_US
dc.identifier.endpage 993 en_US
dc.cauo.name Finance and Economics en_US


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