Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae): testing the influence of a light shift and diel periodicity

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dc.contributor.author Mcginley, Michael en_US
dc.contributor.author Suggett, David en_US
dc.contributor.author Warner, Mark en_US
dc.contributor.editor en_US
dc.date.accessioned 2014-05-18T18:05:43Z
dc.date.available 2014-05-18T18:05:43Z
dc.date.issued 2013 en_US
dc.identifier 2013000110 en_US
dc.identifier.citation Mcginley, Michael, Suggett, David, and Warner, Mark 2013, 'Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae): testing the influence of a light shift and diel periodicity', Journal of Phycology, vol. 49, no. 4, pp. 709-718. en_US
dc.identifier.issn 0022-3646 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/27874
dc.description.abstract Microalgae possess numerous cellular mechanisms specifically employed for acclimating the photosynthetic pathways to changes in the physical environment. Despite the importance of coral-dinoflagellate symbioses, little focus has been given as to how the symbiotic algae (Symbiodinium spp.) regulate the expression of their photosynthetic genes. This study used real-time PCR to investigate the transcript abundance of the plastid-encoded genes, psbA (encoding the D1 protein of photosystem II) and psaA (encoding the P700 protein in photosystem I), within the cultured Symbiodinium ITS-2 (internal transcribed spacer region) types A20 and A13. Transcript abundance was monitored during a low to high-light shift, as well as over a full diel light cycle. In addition, psaA was characterized in three isolates (A20, A13, and D4-5) and noted as another example of a dinoflagellate plastid gene encoded on a minicircle. In general, the overall incongruence of transcript patterns for both psbA and psaA between the Symbiodinium isolates and other models of transcriptionally controlled chloroplast gene expression (e.g., Pisum sativum [pea], Sinapis alba [mustard seedling], and Synechocystis sp. PCC 6803 [cyanobacteria]) suggests that Symbiodinium is reliant on posttranscriptional mechanisms for homeostatic regulation of its photosynthetic proteins. en_US
dc.language en_US
dc.publisher Wiley-blackwell en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/jpy.12079 en_US
dc.title Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae): testing the influence of a light shift and diel periodicity en_US
dc.parent Journal of Phycology en_US
dc.journal.volume 49 en_US
dc.journal.number 4 en_US
dc.publocation Hoboken en_US
dc.identifier.startpage 709 en_US
dc.identifier.endpage 718 en_US
dc.cauo.name SCI.Plant Functional Biology & Climate Change en_US
dc.conference Verified OK en_US
dc.for 060700 en_US
dc.personcode 0000106054 en_US
dc.personcode 120416 en_US
dc.personcode 0000106050 en_US
dc.percentage 100 en_US
dc.classification.name Plant Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords DINOFLAGELLATE KARENIA-BREVIS; REEF CORALS; MICROARRAY ANALYSIS; GONYAULAX-POLYEDRA; PSBA MINICIRCLE; REGULATED GENES; REDOX CONTROL; PHOTOSYNTHESIS; EXPRESSION; ACCLIMATION en_US
dc.staffid en_US


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