Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes

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dc.contributor.author Hanssen, Eric en_US
dc.contributor.author Baum, Jake en_US
dc.contributor.author Riglar, David en_US
dc.contributor.author Rogers, Kelly en_US
dc.contributor.author Cowman, Alan en_US
dc.contributor.author Bullen, Hayley en_US
dc.contributor.author Charnaud, Sarah en_US
dc.contributor.author Przyborski, Jude en_US
dc.contributor.author Gilson, Paul en_US
dc.contributor.author Crabb, Brendan en_US
dc.contributor.author Turnbull, Lynne en_US
dc.contributor.author Whitchurch, Cynthia en_US
dc.contributor.editor en_US
dc.date.accessioned 2013-11-03T17:05:23Z
dc.date.available 2013-11-03T17:05:23Z
dc.date.issued 2013 en_US
dc.identifier 2012001845 en_US
dc.identifier.citation Hanssen, Eric et al. 2013, 'Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes', Nature Publishing Group, vol. 4, pp. 1415 en_US
dc.identifier.issn 2041-1723 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/24040
dc.description.abstract Export of proteins into the infected erythrocyte is critical for malaria parasite survival. The majority of effector proteins are thought to export via a proteinaceous translocon, resident in the parasitophorous vacuole membrane surrounding the parasite. Identification of the Plasmodium translocon of exported proteins and its biochemical association with exported proteins suggests it performs this role. Direct evidence for this, however, is lacking. Here using viable purified Plasmodium falciparum merozoites and three-dimensional structured illumination microscopy, we investigate remodelling events immediately following parasite invasion.We show that multiple complexes of the Plasmodium translocon of exported proteins localize together in foci that dynamically change in clustering behaviour. Furthermore, we provide conclusive evidence of spatial association between exported proteins and exported protein 2, a core component of the Plasmodium translocon of exported proteins, during native conditions and upon generation of translocation intermediates. These data provide the most direct cellular evidence to date that protein export occurs at regions of the parasitophorous vacuole membrane housing the Plasmodium translocon of exported proteins complex. en_US
dc.language en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isbasedon en_US
dc.title Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes en_US
dc.parent Nature Communications en_US
dc.journal.volume 4 en_US
dc.journal.number en_US
dc.publocation United States en_US
dc.identifier.startpage 1415 en_US
dc.identifier.endpage en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060501 en_US
dc.personcode 0000041904 en_US
dc.personcode 0000066102 en_US
dc.personcode 0000085714 en_US
dc.personcode 0000085715 en_US
dc.personcode 0000085777 en_US
dc.personcode 0000091932 en_US
dc.personcode 0000091933 en_US
dc.personcode 0000091934 en_US
dc.personcode 0000091935 en_US
dc.personcode 0000091936 en_US
dc.personcode 103744 en_US
dc.personcode 103745 en_US
dc.percentage 100 en_US
dc.classification.name Bacteriology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords MEROZOITE DENSE GRANULES; HEMOLYSIN-E HLYE; MALARIA PARASITE; HOST-CELL; STRUCTURED ILLUMINATION; MONOCLONAL-ANTIBODIES; BIOLOGICAL-MEMBRANES; PROTEIN IMPORT; CONTACT SITES; INVASION en_US
dc.staffid 103745 en_US


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