Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean

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dc.contributor.author Zhang, Li en_US
dc.contributor.author Wang, Meirong en_US
dc.contributor.author Wang, Chengyin en_US
dc.contributor.author Hu, Xiaoya en_US
dc.contributor.author Wang, Guoxiu en_US
dc.contributor.editor en_US
dc.date.accessioned 2014-04-03T01:22:26Z
dc.date.available 2014-04-03T01:22:26Z
dc.date.issued 2012 en_US
dc.identifier 2012000480 en_US
dc.identifier.citation Wang, Chengyin et al. 2012, 'Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean', Talanta, vol. 101, pp. 226-232. en_US
dc.identifier.issn 0039-9140 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/22792
dc.description.abstract Electrochemical impedance immunosensor, with its high sensitivity from electrochemical impedance analysis and ideal specificity from the immunoassay, is increasingly used in the detection of a kind of phenoxy acid herbicides which is 2,4-Dichlorophenoxybutyric acid (2,4-DB). In this experiment, synthetic 2,4-DB antibodies were immobilized on the electrode by the crosslinking of l-Cysteine/glutaraldehyde, and 2,4-DB were measured by the increase of electron-transfer resistance when the immune reaction occurred, with Fe(CN)63a??/Fe(CN)64a?? as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0??10a??7a??1.0??10a??3 g/L (R=0.994) with the detection limit of 1.0??10a??7 g/L (0.1 ppb). This method bears such merits as simplicity in operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The actual soybean samples were analyzed with the recovery of 82.8%a??102.3%. en_US
dc.language en_US
dc.publisher Elsevier Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.talanta.2012.09.011 en_US
dc.title Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean en_US
dc.parent Talanta en_US
dc.journal.volume 101 en_US
dc.journal.number en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 226 en_US
dc.identifier.endpage 232 en_US
dc.cauo.name SCI.School of Chemistry and Forensic Science en_US
dc.conference Verified OK en_US
dc.for 030100 en_US
dc.personcode 0000087267 en_US
dc.personcode 0000087268 en_US
dc.personcode 119434 en_US
dc.personcode 0000063027 en_US
dc.personcode 109499 en_US
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Immunosensor; 2,4-DB; l-Cysteine; Glutaraldehyde; Soybean en_US
dc.staffid 109499 en_US


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