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Now showing items 21-40 of 939

Use Of Live Attenuated Bacteria To Stimulate Immunity

Fairweather Nf; Chatfield Sn; Charles Ian; Roberts Ms; Lipscombe M; Li Ling-Xi; Strugnell D; Comerford S; Tite J; Dougan G (Editions Scientifiques Elsevier, 1990)

Many bacteria, including Salmonella, Listeria, and Yersinia species, can cause severe systemic infections of mammals. These invasive bacteria appear to have developed sophisticated mechanisms for entry into, and survival ...
Persistent Effects Of Ozone On Needle Water-Loss And Wettability In Norway Spruce

Barnes Jeremy; Eamus Derek; Davison A; Ropoulsen H; Mortensen L (Elsevier Sci Ltd, 1990)

Four-year-old, seed-grown trees of Norway spruce (Picea abies (L.) Karst.) were exposed in open-top chambers to charcoal-filtered air (8 h daily mean 54 ?g O3 m-3) over three consecutive summers (1986¿1988). In mid-May ...
The Role Of A Stress-Response Protein In Bacterial Virulence

Johnson Kylie; Charles Ian; Dougan G; Miller Ia; Pickard D; Ogoara P; Costa G; Ali T; Hormaeche Ce (Editions Scientifiques Elsevier, 1990)

We have utilised the transposon TnphoA to generate Salmonella typhimurium mutants of reduced virulence when given orally to mice (Miller et ai., 1989). S. typhimurium 014 and 046 are two such attenuated derivatiees of the ...
Persistent Stimulation Of Co2 Assimilation And Stomatal Conductance By Summer Ozone Fumigation In Norway Spruce

Eamus Derek; Barnes Jeremy; Mortensen L; Ropoulsen H; Davison A (Elsevier Sci Ltd, 1990)

CO2 assimilation rate, stomatal conductance and chlorophyll content of current and previous years' needles of Norway spruce were measured in May 1988, 205 days after the cessation of ozone fumigation during the summer of ...
A Direct-reading Device For Measurement Of Patch-clamp Micropipette Tip Diameters

Martin Donald; Cook Di (Springer Verlag, 1990)

This paper describes an electronic device for the measurement of the diameter of the tip opening of micropipettes. The device measures the threshold pressure required to produce bubbles from the micropipette submerged in ...
Isolation, Characterization And Nucleotide-Sequences Of The Aroc Genes Encoding Chorismate Synthase From Salmonella-Typhi And Escherichia-Coli

Charles Ian; Lamb Hk; Pickard D; Dougan G; Hawkins Ar (Soc General Microbiology, 1990)

The aroC genes from Salmoneffa @phi and Escherichia cofi, encoding 5-enolpyruvylshikimate-3-phosphate phospholyase (chorismate synthase) were cloned in E. coli and their DNA sequences were determined. The aroC gene from S ...
Complete nucleotide sequence of the aroA gene from Salmonella typhi encoding 5-enolpyruvyishikimate 3-phosphate synthase

Chatfield Steve; Dougan Gordon; Charles Ian (Oxford Univ Press United Kingdom, 1990)

As part of a programme of research to generate fully characterized aro- mutants of Salmonella typhi for use as live attenuated vaccines we have cloned and sequenced the aroC (1) and aroD genes (submitted to J. Gen. Microbiol.) ...
The Structure of a Partial Duplication in the lntegron of Plasmid pDGO100

Hall R; Stokes Harold (Academic Press, 1990)

A family of novel potentially mobile DNA elements, called integrons, has recently been described (H. W. Stokes and R. M. Hall, 1989, Mol. Microbiol. 3, 1669¿1683). The integrons present in the plasmids pDGO100 and pSa are ...
The Influence Of Ozone, Acid Mist And Soil Nutrient Status On Norway Spruce [Picea-Abies (L) Karst] .2. Photosynthesis, Dark Respiration And Soluble Carbohydrates Of Trees During Late Autumn

Barnes Jeremy; Eamus Derek; Brown Kenneth (Cambridge Univ Press, 1990)

Three-year old Norway spruce [Picea abies (L.) Karst.] trees of clonal stock originating from medium altitude in southern Germany were transplanted in May 1986 into two acid brown-earth soils which differed particularly ...
Photosynthetic And Stomatal Conductance Responses Of Norway Spruce And Beech To Ozone, Acid Mist And Frost - A Conceptual-Model

Eamus Derek; Murray Maxine (Elsevier Sci Ltd, 1991)

Two-year-old beech and Norway spruce seedlings were exposed to a combination of ozone and acid mist treatments in open-top chambers in Scotland during the months of July through to September 1988. Replicate pairs of ...
The Hydraulic Conductivity Of Roots Of Rust-Infected Barley Seedlings

Berryman Ca; Eamus Derek; Farrar Jf (Academic Press Ltd, 1991)

The influence of Puccinia hordei (rust) infection on the root hydraulic conductivity (Lp) of excised roots of Hordeum distichum cv. Maris Mink (barley) was investigated using the Scholander pressure chamber technique. The ...
Pertactin, An Arg-Gly-Asp-Containing Bordetella-Pertussis Surface Protein That Promotes Adherence Of Mammalian-Cells

Leininger E; Roberts Ms; Kenimer Jg; Charles Ian; Fairweather N; Novotny P; Brennan Mary (Natl Acad Sciences, 1991)

A 69-kDa protein has been identified on the surface of the Gram-negative pathrogen Bordetella pertussis that can elicit a protective immune response in animal models. This protein is associated with virulent strains of ...
Variations In Epidermal-Cell Turgor Of Rust-Infected Barley Seedlings

Berryman Ca; Eamus Derek; Farrar Jf (Cambridge Univ Press, 1991)

Barley [Hordeum distichum (L.) Lam.] seedlings were infected with urediniospores of rust, Puccinia hordei Otth., 10 d after germination and the Zimmermann micro-pressure probe was used to measure the turgor potential of ...
The commercially available restriction enzyme BspHl is blocked by overlapping methylation

Servos Spiros; Silva C.; Dougan Gordon; Charles Ian (Oxford Univ Press United Kingdom, 1991)

The restriction endonuclease Bspill. conunercially available from New England Biolabs (Bishop's Stortford, UK), cleaves the site T/CATGA. This restriction enzyme is of particular use in the construction of gene expression ...
Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi

Servos Spiros; Chatfield Steve; Hone David; Levine Myron; Dimitriadis George; Pickard Derek; Dougan Gordon; Fairweather Neil; Charles Ian (Soc General Microbiology, 1991)

The aroD gene from Salmonella typhi, encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into Escherichia coli and the DNA sequence determined. The aroD gene was isolated from a cosmid gene bank by ...
High level heterologous expression in E.coli using the anaerobically-activated nirB promoter

Oxer M.; Bentley C.; Doyle J.; Peakman T; Charles Ian; Makoff Andrew (Oxford Univ Press United Kingdom, 1991)

The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell ...
Sulfate And Ammonium In Mist Impair The Frost Hardening Of Red Spruce Seedlings

Cape Jn; Leith Id; Fowler D; Murray Maxine; Sheppard Lj; Eamus Derek; Wilson Rachel (Cambridge Univ Press, 1991)

Two-year-old seedlings of red spruce [Picea rubens Sarg. syn. P. rubra (Du Roi) Link] were grown in open-top chambers supplied with charcoal-filtered air near Edinburgh, Scotland. Between May and November 1988, plants ...
Sequence Analysis of the Inducible Chloramphenicol Resistance Determinant in the Tn7696 lntegron Suggests Regulation by Translational Attenuation

Stokes Harold; Hall R (Academic Press, 1991)

The sequence of the Tn1696 determinant for inducible nonenzymatic chloramphenicol resistance has been determined. The cml region, the fourth insert of the Tn1696 integron, is 1547 bases and includes a 59-base element at ...
A new trimethoprim resistance gene, dhfrX, in the In 7 integron of plasmid pDGO100

Parsons Yvonne; Hall Ruth; Stokes Harold (American Society for Microbiology, 1991)

A new trimethoprim resistance determinant, designated dhfrX, was identified in the In7 integron of pDGO100. The sequence of the dhfrX dihydrofolate reductase is up to 28% identical to the sequences of several known ...
Water Relations Of Leaves Of Barley Infected With Brown Rust

Berryman Ca; Eamus Derek; Farrar Jf (Academic Press Ltd, 1991)

The water balance of brown rust (Puccinia hordei) infected barley seedlings was investigated. It was found that with the progression of the disease, the infected leaves lost their ability to maintain a favourable water ...

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