Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in P2X7 receptor-deficient mice

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dc.contributor.author Miller, Catherine en_US
dc.contributor.author Zakrzewski, Alana en_US
dc.contributor.author Ikin, Rowan en_US
dc.contributor.author Boulter, Nicola en_US
dc.contributor.author Katrib, Marilyn en_US
dc.contributor.author Lees, Michael en_US
dc.contributor.author Fuller, Stephen en_US
dc.contributor.author Wiley, James en_US
dc.contributor.author Smith, Narelle en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:34:20Z
dc.date.available 2012-10-12T03:34:20Z
dc.date.issued 2011 en_US
dc.identifier 2010004737 en_US
dc.identifier.citation Miller Catherine et al. 2011, 'Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in P2X7 receptor-deficient mice', Elsevier Sci Ltd, vol. 41, no. 3-4, pp. 301-308. en_US
dc.identifier.issn 0020-7519 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18531
dc.description.abstract The P2X(7) receptor (P2X(7)R) is a two transmembrane receptor that is highly expressed on the surface of immune cells. Loss of function polymorphisms in this receptor have been linked to increased susceptibility to intracellular pathogens. P2X7R gene knockout (P2X(7)R(-/-); on a C57Bl/6J background), C57Bl/6J and BALB/c mice were infected with the avirulent ME49 strain of the intracellular parasite, Toxoplasma gondii. and susceptibility determined by monitoring weight loss. P2X7R(-/-) mice lost significantly more weight than C57Bl/6J mice from day 8 p.i. C57Bl/6J, in turn, lost significantly more weight than BALB/c mice. Thus, by day 10 p.i., P2X(7)R(-/-) mice had lost 5.7 +/- 0.7% of their weight versus 2.4 +/- 0.6% for C57Bl/6J mice, whereas BALB/c mice had gained 1.9 +/- 0.5%; by day 12 p.i., P2X(7)R(-/-) mice had lost 15.1 +/- 0.6%, C57Bl/6J had lost 10.1 +/- 0.8% and BALB/c had lost 4.8 +/- 0.8% of their weight. Neither parasite burden nor liver pathology was greater in the P2X(7)R(-/-) mice than in C57Bl/6J mice but BALB/c mice had significantly smaller numbers of parasites and less pathology in their livers than these strains. Absence of the P2X7 receptor did not affect IFN-gamma, IL-12, IL-1 beta, monocyte chemoattractant protein-1 (MCP-1) or TNF production. However, both P2X(7)R(-/-) and C57Bl/6J mice produced more IL-1 beta and INF than BALB/c mice. There was one important point of differentiation between the P2X(7)R(-/-) and C57Bl/6J mice, namely the significantly enhanced and prolonged production of nitric oxide, accompanied by delayed production of IL-10 in the P2X(7)R-deficient mice. en_US
dc.language en_US
dc.publisher Elsevier Sci Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.ijpara.2010.10.001 en_US
dc.title Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in P2X7 receptor-deficient mice en_US
dc.parent International Journal For Parasitology en_US
dc.journal.volume 41 en_US
dc.journal.number 3-4 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 301 en_US
dc.identifier.endpage 308 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 970043 en_US
dc.personcode 100992 en_US
dc.personcode 103482 en_US
dc.personcode 011172 en_US
dc.personcode 044726 en_US
dc.personcode 044579 en_US
dc.personcode 0000020556 en_US
dc.personcode 0000020554 en_US
dc.personcode 921144 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity WOS:000288736700005 en_US
dc.description.keywords Phospholipase-D Activation; Nf-Kappa-B; Human Macrophages; Human Monocytes; Cell-Death; Mycobacterium-Tuberculosis; Murine Susceptibility; Signaling Pathways; P2Z Purinoreceptor; Peroral Infection en_US
dc.staffid 921144 en_US

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