Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms

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dc.contributor.author Staal, M. en_US
dc.contributor.author Borisov, S. en_US
dc.contributor.author Rickelt, Lars en_US
dc.contributor.author Klimant, Ingo en_US
dc.contributor.author Kuhl, Michael en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:34:07Z
dc.date.available 2012-10-12T03:34:07Z
dc.date.issued 2011 en_US
dc.identifier 2010002506 en_US
dc.identifier.citation Staal M. et al. 2011, 'Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms', Elsevier, vol. 85, no. 1, pp. 67-74. en_US
dc.identifier.issn 0167-7012 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18434
dc.description.abstract New transparent optodes for life-time based microscopic imaging of O-2 were developed by spin-coating a mu m-thin layer of a highly luminescent cyclometalated iridium(III) coumarin complex in polystyrene onto glass cover slips. Compared to similar thin-film O-2 optodes based on a ruthenium(II) polypyridyl complex or a platinum(II) porphyrin, the new planar sensors have i) higher brightness allowing for much shorter exposure times and thus higher time resolution, ii) more homogeneous and smaller pixel to pixel variation over the sensor area resulting in less noisy O-2 images, and iii) a lower temperature dependency simplifying calibration procedures. We used the new optodes for microscopic imaging of the spatio-temporal O-2 dynamics at the base of heterotrophic biofilms in combination with confocal imaging of bacterial biomass and biofilm structure. This allowed us to directly link biomass distribution to O-2 distribution under both steady state and non-steady state conditions. We demonstrate that the O-2 dynamics in biofilms i; governed by a complex interaction between biomass distribution, mass transfer and flow that cannot be directly inferred from structural information on biomass distribution alone. en_US
dc.language en_US
dc.publisher Elsevier en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.mimet.2011.01.021 en_US
dc.title Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms en_US
dc.parent Journal of Microbiological Methods en_US
dc.journal.volume 85 en_US
dc.journal.number 1 en_US
dc.publocation Amsterdam en_US
dc.identifier.startpage 67 en_US
dc.identifier.endpage 74 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 100703 en_US
dc.personcode 0000061189 en_US
dc.personcode 0000067497 en_US
dc.personcode 0000061178 en_US
dc.personcode 0000062480 en_US
dc.personcode 107129 en_US
dc.percentage 100 en_US
dc.classification.name Nanobiotechnology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Biofilm; Imaging; Luminescence; Microscopy; Oxygen; Optode en_US
dc.staffid en_US
dc.staffid 107129 en_US

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