Oocysts and high seroprevalence of Neospora caninum in dogs living in remote Aboriginal communities and wild dogs in Australia

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dc.contributor.author King, Jess en_US
dc.contributor.author Brown, Graeme en_US
dc.contributor.author Jenkins, David en_US
dc.contributor.author Ellis, John en_US
dc.contributor.author Fleming, Peter en_US
dc.contributor.author Windsor, Peter en_US
dc.contributor.author Slapeta, Jan en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:33:37Z
dc.date.available 2012-10-12T03:33:37Z
dc.date.issued 2012 en_US
dc.identifier 2010003702 en_US
dc.identifier.citation King Jess et al. 2012, 'Oocysts and high seroprevalence of Neospora caninum in dogs living in remote Aboriginal communities and wild dogs in Australia', Elsevier Inc, vol. 187, no. 1-2, pp. 85-92. en_US
dc.identifier.issn 0304-4017 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18210
dc.description.abstract Canines are definitive hosts of Neospora caninum (Apicomplexa). For horizontal transmission from canines to occur, viable oocysts of N. caninum must occur in the environment of susceptible intermediate hosts. Canids in Australia include wild dogs and Aboriginal community dogs. Wild dogs are those dogs that are not dependent on humans for survival and consist of the dingo, feral domestic dog and their hybrid genotypes. Aboriginal community dogs are dependent on humans, domesticated and owned by a family, but are free-roaming and have free access throughout the community. In this study the extent of N. caninum infection was determined in a total of 374 dogs (75 wild dogs and 299 Aboriginal community dogs) using a combination of microscopic, molecular and serological techniques. Oocysts of N. caninum were observed in the faeces of two juvenile Aboriginal community dogs (2/132; 1.5%). To estimate N. caninum prevalence, a new optimised cut-off of 18.5% inhibition for a commercial competitive ELISA was calculated using a two-graph receiver-operating characteristic (TG-ROC) analysis and IFAT as the gold standard resulting in equal sensitivity and specificity of 67.8%. Of the 263 dog sera tested the true prevalence of N. caninum antibodies was 27.0% (95% confidence limit: 10.3-44.1%). The association between the competitive ELISA results in dogs less than 12 month old and older dogs was significant (P=0.042). To our knowledge this is the first large scale parasitological survey of the Aboriginal community dogs and wild dogs from Australia. The high prevalence of N. canilium infection in Aboriginal community dogs illustrates that horizontal transmission of N. caninum is occurring in Australia. These results demonstrated that N. caninum in dogs is widespread, including the semi-arid to arid regions of north-western New South Wales and the Northern Territory. The populations of free-ranging dogs are likely to be important contributors to the sylvatic life cycle of N. caninum. en_US
dc.language en_US
dc.publisher Elsevier Inc en_US
dc.relation.isbasedon http://dx.doi.org/10.1016/j.vetpar.2011.12.027 en_US
dc.title Oocysts and high seroprevalence of Neospora caninum in dogs living in remote Aboriginal communities and wild dogs in Australia en_US
dc.parent Veterinary Parasitology en_US
dc.journal.volume 187 en_US
dc.journal.number 1-2 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 85 en_US
dc.identifier.endpage 92 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 070708 en_US
dc.personcode 0000061081 en_US
dc.personcode 0000070752 en_US
dc.personcode 0000053777 en_US
dc.personcode 910945 en_US
dc.personcode 0000070753 en_US
dc.personcode 0000022414 en_US
dc.personcode 0000052877 en_US
dc.percentage 100 en_US
dc.classification.name Veterinary Parasitology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Neosporosis; Dog; Oocysts; ELISA; IFAT; Serology; Epidemiology en_US

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