Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella

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dc.contributor.author Behrendt, Lars en_US
dc.contributor.author Larkum, Anthony en_US
dc.contributor.author Trampe, Erik en_US
dc.contributor.author Norman, Anders en_US
dc.contributor.author S??Rensen, S??Ren en_US
dc.contributor.author Kuhl, Michael en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:33:33Z
dc.date.available 2012-10-12T03:33:33Z
dc.date.issued 2012 en_US
dc.identifier 2011004057 en_US
dc.identifier.citation Behrendt Lars et al. 2012, 'Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella', Nature Publishing Group, vol. 6, no. 6, pp. 1222-1237. en_US
dc.identifier.issn 1751-7362 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18206
dc.description.abstract We assessed the microbial diversity and microenvironmental niche characteristics in the didemnid ascidian Lissoclinum patella using 16S rRNA gene sequencing, microsensor and imaging techniques. L. patella harbors three distinct microbial communities spatially separated by few millimeters of tunic tissue: (i) a biofilm on its upper surface exposed to high irradiance and O-2 levels, (ii) a cloacal cavity dominated by the prochlorophyte Prochloron spp. characterized by strong depletion of visible light and a dynamic chemical microenvironment ranging from hyperoxia in light to anoxia in darkness and (iii) a biofilm covering the underside of the animal, where light is depleted of visible wavelengths and enriched in near-infrared radiation (NIR). Variable chlorophyll fluorescence imaging demonstrated photosynthetic activity, and hyperspectral imaging revealed a diversity of photopigments in all microhabitats. Amplicon sequencing revealed the dominance of cyanobacteria in all three layers. Sequences representing the chlorophyll d containing cyanobacterium Acaryochloris marina and anoxygenic phototrophs were abundant on the underside of the ascidian in shallow waters but declined in deeper waters. This depth dependency was supported by a negative correlation between A. marina abundance and collection depth, explained by the increased attenuation of NIR as a function of water depth. The combination of microenvironmental analysis and fine-scale sampling techniques used in this investigation gives valuable first insights into the distribution, abundance and diversity of bacterial communities associated with tropical ascidians. In particular, we show that microenvironments and microbial diversity can vary significantly over scales of a few millimeters in such habitats; which is information easily lost by bulk sampling en_US
dc.language en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isbasedon http://dx.doi.org/10.1038/ismej.2011.181 en_US
dc.title Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella en_US
dc.parent ISME Journal en_US
dc.journal.volume 6 en_US
dc.journal.number 6 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 1222 en_US
dc.identifier.endpage 1237 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000067754 en_US
dc.personcode 111269 en_US
dc.personcode 0000067758 en_US
dc.personcode 0000067755 en_US
dc.personcode 0000067757 en_US
dc.personcode 107129 en_US
dc.percentage 34 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Acaryochloris marina; Lissoclinum patella; microenvironment; microbial diversity; microsensor; Prochloron en_US
dc.staffid en_US
dc.staffid 107129 en_US

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