Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs

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dc.contributor.author Earp, Alan en_US
dc.contributor.author Hanson, Christine en_US
dc.contributor.author Ralph, Peter en_US
dc.contributor.author Brando, Vittorio en_US
dc.contributor.author Allen, Simon en_US
dc.contributor.author Baird, Mark en_US
dc.contributor.author Clementson, Lesley en_US
dc.contributor.author Daniel, Paul en_US
dc.contributor.author Dekker, Arnold en_US
dc.contributor.author Fearns, Peter en_US
dc.contributor.author Parslow, J en_US
dc.contributor.author Strutton, Peter en_US
dc.contributor.author Thompson, Peter en_US
dc.contributor.author Underwood, Mark en_US
dc.contributor.author Weeks, Scarla en_US
dc.contributor.author Doblin, Martina en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:33:29Z
dc.date.available 2012-10-12T03:33:29Z
dc.date.issued 2011 en_US
dc.identifier 2010007011 en_US
dc.identifier.citation Earp Alan et al. 2011, 'Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs', Optical Society of America, vol. 19, no. 27, pp. 26768-26782. en_US
dc.identifier.issn 1094-4087 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18163
dc.description.abstract Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, but measurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescent materials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometera??s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit a variety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeated measurements with the recommended solid standard, Plexiglas Satinice? plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors. en_US
dc.language en_US
dc.publisher Optical Society of America en_US
dc.rights This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.19.026768. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law."
dc.title Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs en_US
dc.parent Optics Express en_US
dc.journal.volume 19 en_US
dc.journal.number 27 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 26768 en_US
dc.identifier.endpage 26782 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 060200 en_US
dc.personcode 014240 en_US
dc.personcode 0000072143 en_US
dc.personcode 890085 en_US
dc.personcode 0000072144 en_US
dc.personcode 0000072145 en_US
dc.personcode 108934 en_US
dc.personcode 0000074151 en_US
dc.personcode 0000074152 en_US
dc.personcode 0000072146 en_US
dc.personcode 0000072147 en_US
dc.personcode 0000063613 en_US
dc.personcode 0000072148 en_US
dc.personcode 0000028439 en_US
dc.personcode 0000072149 en_US
dc.personcode 0000072150 en_US
dc.personcode 996445 en_US
dc.percentage 100 en_US
dc.classification.name Ecology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords DISSOLVED ORGANIC-MATTER; ABSORPTION; SENSORS en_US
dc.staffid 996445 en_US


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