The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli

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dc.contributor.author Ferenci, Ferenci en_US
dc.contributor.author Galbiati, Heloisa en_US
dc.contributor.author Betteridge, Thu en_US
dc.contributor.author Phan, Katherine en_US
dc.contributor.author Spira, Beny en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-10-12T03:33:25Z
dc.date.available 2012-10-12T03:33:25Z
dc.date.issued 2011 en_US
dc.identifier 2010004548 en_US
dc.identifier.citation Ferenci Ferenci et al. 2011, 'The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli', BioMed Central, vol. 11, no. 62, pp. 1-11. en_US
dc.identifier.issn 1471-2180 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/18132
dc.description.abstract Background Sigma factors and the alarmone ppGpp control the allocation of RNA polymerase to promoters under stressful conditions. Both ppGpp and the sigma factor sS (RpoS) are potentially subject to variability across the species Escherichia coli. To find out the extent of strain variation we measured the level of RpoS and ppGpp using 31 E. coli strains from the ECOR collection and one reference K-12 strain. Results Nine ECORs had highly deleterious mutations in rpoS, 12 had RpoS protein up to 7-fold above that of the reference strain MG1655 and the remainder had comparable or lower levels. Strain variation was also evident in ppGpp accumulation under carbon starvation and spoT mutations were present in several low-ppGpp strains. Three relationships between RpoS and ppGpp levels were found: isolates with zero RpoS but various ppGpp levels, strains where RpoS levels were proportional to ppGpp and a third unexpected class in which RpoS was present but not proportional to ppGpp concentration. High-RpoS and high-ppGpp strains accumulated rpoS mutations under nutrient limitation, providing a source of polymorphisms. Conclusions The ppGpp and sS variance means that the expression of genes involved in translation, stress and other traits affected by ppGpp and/or RpoS are likely to be strain-specific and suggest that influential components of regulatory networks are frequently reset by microevolution. Different strains of E. coli have different relationships between ppGpp and RpoS levels and only some exhibit a proportionality between increasing ppGpp and RpoS levels as demonstrated for E. coli K-12. en_US
dc.language en_US
dc.publisher BioMed Central en_US
dc.title The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli en_US
dc.parent BMC Microbiology en_US
dc.journal.volume 11 en_US
dc.journal.number 62 en_US
dc.publocation United States en_US
dc.identifier.startpage 1 en_US
dc.identifier.endpage 11 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060500 en_US
dc.personcode 0000053989 en_US
dc.personcode 0000076346 en_US
dc.personcode 106539 en_US
dc.personcode 0000076347 en_US
dc.personcode 0000076348 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Na en_US


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