The androgen receptor drives the sex-specific expression of vascular cell adhesion molecule-1 in endothelial cells but not lipid metabolism genes in monocyte-derived macrophages

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dc.contributor.author Mcgrath, Kristine en_US
dc.contributor.author Hill, Michelle en_US
dc.contributor.author Mcrobb, Lucinda en_US
dc.contributor.author Heather, Alison en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-03-12T11:23:35Z
dc.date.available 2012-03-12T11:23:35Z
dc.date.issued 2010 en_US
dc.identifier 2010001852 en_US
dc.identifier.citation Mcgrath Kristine et al. 2010, 'The androgen receptor drives the sex-specific expression of vascular cell adhesion molecule-1 in endothelial cells but not lipid metabolism genes in monocyte-derived macrophages', Walter de Gruyter GmbH & Co. KG, vol. 2, no. 1, pp. 203-209. en_US
dc.identifier.issn 1868-1883 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/17556
dc.description.abstract Background: Anecdotal evidence suggests that male sex hormones are proatherogenic. We hypothesized that the male sex hormone receptor, the androgen receptor (AR), acts as a molecular switch in sex-specific inflammatory signaling in vascular cells.Materials and methods: AR expression in human umbilical vein endothelial cells (HUVECs), human monocyte-derived macrophages (MDMs) or HeLa cells was modulated by transfection with AR siRNA or human AR cDNA expression vector. Activity and expression levels were measured by luciferase reporter assays, Western blotting or real-time PCR analysis.Results: AR knockdown reduced expression of vascular cell adhesion molecule-1 (VCAM-1) in genetically male HUVECs. Conversely, AR upregulation in genetically female HUVECs induced VCAM-1 expression and increased dihydrotestosterone-stimulated monocyte adhesion. Co-transfection of an AR expression vector with VCAM-1 or NF-?B-reporter vectors into phenotypically female, AR-negative HeLa cells confirmed AR regulation of VCAM-1 expression as well as AR activation of NF-?B. AR upregulation was not sufficient to increase ICAM-1 levels in female HUVECs or lipoprotein metabolism gene expression in female MDMs, despite AR knockdown limiting expression in their male counterparts. en_US
dc.language en_US
dc.publisher Walter de Gruyter GmbH & Co. KG en_US
dc.rights The final publication is available at www.degruyter.com
dc.title The androgen receptor drives the sex-specific expression of vascular cell adhesion molecule-1 in endothelial cells but not lipid metabolism genes in monocyte-derived macrophages en_US
dc.parent Hormone Molecular Biology and Clinical Investigation en_US
dc.journal.volume 2 en_US
dc.journal.number 1 en_US
dc.publocation Germany en_US
dc.identifier.startpage 203 en_US
dc.identifier.endpage 209 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 110800 en_US
dc.personcode 111077 en_US
dc.personcode 0000066710 en_US
dc.personcode 0000065911 en_US
dc.personcode 108123 en_US
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords androgen receptor, inflammation, nuclear factor-?B, vascular cell adhesion molecule-1 en_US
dc.staffid 108123 en_US


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