P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages

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dc.contributor.author Lees, Michael en_US
dc.contributor.author Fuller, Stephen en_US
dc.contributor.author Mcleod, R en_US
dc.contributor.author Boulter, Nicola en_US
dc.contributor.author Miller, Catherine en_US
dc.contributor.author Zakrzewski, Alana en_US
dc.contributor.author Mui, Ej en_US
dc.contributor.author Witola, William en_US
dc.contributor.author Coyne, Jj en_US
dc.contributor.author Hargrave, Ac en_US
dc.contributor.author Jamieson, S en_US
dc.contributor.author Blackwell, J en_US
dc.contributor.author Wiley, James en_US
dc.contributor.author Smith, Nicholas en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-10T06:09:48Z
dc.date.available 2012-02-10T06:09:48Z
dc.date.issued 2011 en_US
dc.identifier 2011000578 en_US
dc.identifier.citation Lees Michael et al. 2011, 'P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages', American Association of Immunologists, vol. 184, no. 12, pp. 7040-7046. en_US
dc.identifier.issn 0022-1767 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/17140
dc.description.abstract The P2X7R is highly expressed on the macrophage cell surface, and activation of infected cells by extracellular ATP has been shown to kill intracellular bacteria and parasites. Furthermore, single nucleotide polymorphisms that decrease receptor function reduce the ability of human macrophages to kill Mycobacterium tuberculosis and are associated with extrapulmonary tuberculosis. In this study, we show that macrophages from people with the 1513C (rs3751143, NM-002562.4:c.1487A>C) loss-of-function P2X 7R single nucleotide polymorphism are less effective in killing intracellular Toxoplasma gondii after exposure to ATP compared with macrophages from people with the 1513A wild-type allele. Supporting a P2X 7R-specific effect on T. gondii, macrophages from P2X7R knockout mice (P2X7R-/-) are unable to kill T. gondii as effectively as macrophages from wild-type mice. We show that P2X 7R-mediated T. gondii killing occurs in parallel with host cell apoptosis and is independent of NO production en_US
dc.language en_US
dc.publisher American Association of Immunologists en_US
dc.relation.isbasedon http://dx.doi.org/10.4049/jimmunol.1000012 en_US
dc.title P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages en_US
dc.parent Journal of Immunology en_US
dc.journal.volume 184 en_US
dc.journal.number 12 en_US
dc.publocation USA en_US
dc.identifier.startpage 7040 en_US
dc.identifier.endpage 7046 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 110700 en_US
dc.personcode 044579 en_US
dc.personcode 0000020556 en_US
dc.personcode 0000067922 en_US
dc.personcode 011172 en_US
dc.personcode 970043 en_US
dc.personcode 100992 en_US
dc.personcode 0000067595 en_US
dc.personcode 0000072838 en_US
dc.personcode 0000067602 en_US
dc.personcode 0000067593 en_US
dc.personcode 0000061837 en_US
dc.personcode 0000060219 en_US
dc.personcode 0000020554 en_US
dc.personcode 960087 en_US
dc.percentage 100 en_US
dc.classification.name Immunology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords adenosine triphosphate; purinergic P2X7 receptor; nitric oxide; purinergic P2 receptor en_US
dc.staffid 960087 en_US

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