Kavalactones yangonin and methysticin induce apoptosis in human hepatocytes (HepG2) in vitro

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dc.contributor.author Tang, J. en_US
dc.contributor.author Dunlop, Rachael en_US
dc.contributor.author Rowe, A. en_US
dc.contributor.author Ramzan, I. en_US
dc.contributor.author Rodgers, Ken en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T11:01:06Z
dc.date.available 2012-02-02T11:01:06Z
dc.date.issued 2011 en_US
dc.identifier 2010001151 en_US
dc.identifier.citation Tang J. et al. 2011, 'Kavalactones yangonin and methysticin induce apoptosis in human hepatocytes (HepG2) in vitro', John Wiley and Sons, vol. 25, no. 3, pp. 417-423. en_US
dc.identifier.issn 0951-418X en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15608
dc.description.abstract While cases of severe kava hepatotoxicity have been reported, studies examining the toxicity of individual kavalactones are limited. The present study examined the in vitro hepatotoxicity of kavain, methysticin and yangonin on human hepatocytes (HepG2) and the possible mechanism(s) involved. Cytotoxicity was assessed using lactate dehydrogenase (LDH) and ethidium bromide (EB) assays. The mode of cell death was analysed with acridine orange/ethidium bromide dual staining with fluorescence microscopy. Glutathione oxidation was measured using the ortho-phthalaldehyde (OPT) fluorescence assay. Kavain had minimal cytotoxicity, methysticin showed moderate concentration-dependent toxicity and yangonin displayed marked toxicity with ~40% reduction in viability in the EB assay. Acridine orange/ethidium bromide staining showed the predominant mode of cell death was apoptosis rather than necrosis. No significant changes were observed in glutathione levels, excluding this as the primary mechanism of cell death in this model. Further studies may elucidate the precise apoptotic pathways responsible and whether toxic kavalactone metabolites are involved. en_US
dc.language en_US
dc.publisher John Wiley and Sons en_US
dc.relation.isbasedon http://dx.doi.org/10.1002/ptr.3283 en_US
dc.title Kavalactones yangonin and methysticin induce apoptosis in human hepatocytes (HepG2) in vitro en_US
dc.parent Phytotherapy Research en_US
dc.journal.volume 25 en_US
dc.journal.number 3 en_US
dc.publocation United States en_US
dc.identifier.startpage 417 en_US
dc.identifier.endpage 423 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 111500 en_US
dc.personcode 0000066092 en_US
dc.personcode 115899 en_US
dc.personcode 0000066094 en_US
dc.personcode 111642 en_US
dc.personcode 0000066095 en_US
dc.percentage 100 en_US
dc.classification.name Pharmacology and Pharmaceutical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords cytotoxicity;kavalactone;kavain;methysticin;yangonin;apoptosis;HepG2;hepatocyte en_US

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