Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial mat

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dc.contributor.author Steunou, Anne-Soisig en_US
dc.contributor.author Jensen, Sheila en_US
dc.contributor.author Brecht, Eric en_US
dc.contributor.author Becraft, Eric en_US
dc.contributor.author Bateson, Mary en_US
dc.contributor.author Kilian, Oliver en_US
dc.contributor.author Bhaya, Devaki en_US
dc.contributor.author Ward, David en_US
dc.contributor.author Peters, John en_US
dc.contributor.author Grossman, Arthur en_US
dc.contributor.author Kuhl, Michael en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T10:57:42Z
dc.date.available 2012-02-02T10:57:42Z
dc.date.issued 2008 en_US
dc.identifier 2009004080 en_US
dc.identifier.citation Steunou Anne-Soisig et al. 2008, 'Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial mat', Nature Publishing Group, vol. 2, no. 4, pp. 364-378. en_US
dc.identifier.issn 1751-7362 en_US
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15468
dc.description.abstract Nitrogen fixation, a prokaryotic, O2-inhibited process that reduces N2 gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night, and only declined when the mat became oxic in the morning. Nitrogenase activity was low throughout the night; however, it exhibited two peaks, a small one in the evening and a large one in the early morning, when light began to stimulate cyanobacterial photosynthetic activity, but O2 consumption by respiration still exceeded the rate of O2 evolution. Once the irradiance increased to the point at which the mat became oxic, the nitrogenase activity was strongly inhibited. en_US
dc.language en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isbasedon http://dx.doi.org/10.1038/ismej.2007.117 en_US
dc.title Regulation of nif gene expression and the energetics of N2 fixation over the diel cycle in a hot spring microbial mat en_US
dc.parent ISME Journal en_US
dc.journal.volume 2 en_US
dc.journal.number 4 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 364 en_US
dc.identifier.endpage 378 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 100200 en_US
dc.personcode 0000061230 en_US
dc.personcode 0000061187 en_US
dc.personcode 0000061231 en_US
dc.personcode 0000061232 en_US
dc.personcode 0000061233 en_US
dc.personcode 0000061234 en_US
dc.personcode 0000061235 en_US
dc.personcode 0000061236 en_US
dc.personcode 0000061237 en_US
dc.personcode 0000062169 en_US
dc.personcode 107129 en_US
dc.percentage 100 en_US
dc.classification.name Environmental Biotechnology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Synechococcus, microsensor, gene expression, nitrogen fixation, Yellowstone National Park, in situ en_US
dc.staffid 107129 en_US


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