Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae nind heparin fibronectin and procine cilia

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dc.contributor.author Deutscher, Ania en_US
dc.contributor.author Jenkins, Cheryl en_US
dc.contributor.author Minion, F. Chris en_US
dc.contributor.author Seymour, L en_US
dc.contributor.author Dixon, Nicholas en_US
dc.contributor.author Walker, M en_US
dc.contributor.author Djordjevic, Steven en_US
dc.contributor.author Padula, Matt en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T08:42:19Z
dc.date.available 2012-02-02T08:42:19Z
dc.date.issued 2010 en_US
dc.identifier 2009006780 en_US
dc.identifier.citation Deutscher Ania et al. 2010, 'Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae nind heparin fibronectin and procine cilia', Blackwell Publishing Ltd, vol. 78, no. 2, pp. 444-458. en_US
dc.identifier.issn 0950-382X en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/15028
dc.description.abstract Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, adheres to ciliated respiratory epithelia resulting in ciliostasis and epithelial cell death. The cilium adhesin P97 (Mhp183) contains two repeat regions, designated R1 and R2, that play key roles in adherence. Eight pentapeptide repeats in R1 are sufficient to bind porcine cilia; however, both R1 and R2 are needed to bind heparin. Mhp271, a paralogue of P97, is the only other M. hyopneumoniae protein to contain both R1 and R2 repeats. These repeats are arranged as a set of three pentapeptide repeats (designated R1A271), two decapeptide repeats (designated R2271), and a second set of six pentapeptide repeats (designated R1B271). To determine their function, recombinant proteins containing R1A271 (F1271) and R2271-R1B271 (F2271) were constructed and used in in vitro binding assays. F2271, but not F1271, bound heparin (KD = 8.1 ? 0.4 nM), fibronectin (KD = 174 ? 13 nM) and porcine cilia. Pre-incubation of F2271 with 100 ?M heparin blocked cilium binding by ?69%. Cell surface shaving with trypsin combined with two-dimensional liquid chromatography coupled to tandem mass spectrometry analysis identified Mhp271 as surface-exposed. Our data suggest that both R1 and R2 in Mhp271 are involved in binding to host molecules en_US
dc.language en_US
dc.publisher Blackwell Publishing Ltd en_US
dc.relation.isbasedon http://dx.doi.org/10.1111/j.1365-2958.2010.07345.x en_US
dc.title Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae nind heparin fibronectin and procine cilia en_US
dc.parent Molecular Microbiology en_US
dc.journal.volume 78 en_US
dc.journal.number 2 en_US
dc.publocation UK en_US
dc.identifier.startpage 444 en_US
dc.identifier.endpage 458 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 070700 en_US
dc.personcode 0000063302 en_US
dc.personcode 117836 en_US
dc.personcode 0000052258 en_US
dc.personcode 0000063306 en_US
dc.personcode 970082 en_US
dc.personcode 0000062457 en_US
dc.personcode 0000052539 en_US
dc.personcode 107126 en_US
dc.percentage 100 en_US
dc.classification.name Veterinary Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.staffid en_US
dc.staffid 107126 en_US


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