In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS

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dc.contributor.author Jensen, Sheila en_US
dc.contributor.author Steunou, Anne-Soisig en_US
dc.contributor.author Bhaya, Devaki en_US
dc.contributor.author Kuhl, Michael en_US
dc.contributor.author Grossman, Arthur en_US
dc.contributor.editor en_US
dc.date.accessioned 2012-02-02T07:41:38Z
dc.date.available 2012-02-02T07:41:38Z
dc.date.issued 2011 en_US
dc.identifier 2010000247 en_US
dc.identifier.citation Jensen Sheila et al. 2011, 'In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS', Nature Publishing Group, vol. 5, no. 2, pp. 317-328. en_US
dc.identifier.issn 1751-7362 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/14909
dc.description.abstract The relative abundance of transcripts encoding proteins involved in inorganic carbon concentrating mechanisms (CCM), detoxification of reactive oxygen species (ROS) and photosynthesis in the thermophilic cyanobacterium Synechococcus OS-B0 was measured in hot spring microbial mats over two diel cycles, and was coupled with in situ determinations of incoming irradiance and microenvironmental dynamics of O2 and pH. Fluctuations in pH and O2 in the mats were largely driven by the diel cycle of solar irradiance, with a pH variation from B7.0 to B9.5, and O2 levels ranging from anoxia to supersaturation during night and day, respectively. Levels of various transcripts from mat cyanobacteria revealed several patterns that correlated with incident irradiance, O2 and pH within the mat matrix. Transcript abundances for most genes increased during the morning darka??light transition. Some transcripts remained at a near constant level throughout the light period, whereas others showed an additional increase in abundance as the mat underwent transition from low-to-high light (potentially reflecting changes in O2 concentration and pH), followed by either a decreased abundance in the early afternoon, or a gradual decline during the early afternoon and into the evening. One specific transcipt, psbA1, was the lowest during mid-day under high irradiance and increased when the light levels declined. We discuss these complex in situ transcriptional patterns with respect to environmental and endogenous cues that might impact and regulate transcription over the diel cycle. en_US
dc.language en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isbasedon http://dx.doi.org/10.1038/ismej.2010.131 en_US
dc.title In situ dynamics of O2, pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS en_US
dc.parent ISME Journal en_US
dc.journal.volume 5 en_US
dc.journal.number 2 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 317 en_US
dc.identifier.endpage 328 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060200 en_US
dc.personcode 0000061187 en_US
dc.personcode 0000061230 en_US
dc.personcode 0000061235 en_US
dc.personcode 107129 en_US
dc.personcode 0000062169 en_US
dc.percentage 50 en_US
dc.classification.name Ecology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords CCM, ROS, cyanobacteria, hot springs, gene expression, microsensor, photosynthesis, biofilms en_US


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